l-Alpha-glycerylphosphorylcholine can be cytoprotective or cytotoxic in neonatal rat cardiac myocytes: a double-edged sword phenomenon

Eszter Tuboly, Renáta Gáspár, Miguel Olias Ibor, Kamilla Gömöri, Bernadett Kiss, Gerda Strifler, P. Hartmann, Péter Ferdinandy, Monika Bartekova, M. Borós, A. Görbe

Research output: Contribution to journalArticle

Abstract

l-Alpha-glycerylphosphorylcholine (GPC) is a widely used food supplement. GPC has been shown to exert beneficial effects in several organs; however, the cardiac effects of GPC have yet to be investigated. The aim of the present study was therefore to map out the effects of GPC on cardiac myocytes, with or without ischemia–reperfusion insult. Neonatal rat cardiac myocytes were treated with GPC at 1, 10, 80, and 100 µM concentrations for 15 min, 3 h, or 24 h, respectively. Cell viability by calcein assay and the degree of oxidative stress by DHE (superoxide level) and H2DCF (total ROS accumulation) staining were measured. In separate experiments, cardiomyocytes were pre-treated with the optimal concentration of GPC for 3 h and then cells were exposed to 4 h of simulated ischemia followed by 2 h of reperfusion (SI/R). Cell viability was measured at the end of the SI/R protocol. In normoxic conditions, the 15-min and the 3-h GPC treatment did not affect cell viability, total ROS, and superoxide levels. Under SI/R conditions, the 3-h GPC treatment protected the cardiac myocytes from SI/R-induced cell death and did not alter the level of oxidative stress. The 24-h GPC treatment in normoxic conditions resulted in significant cell death and increased oxidative stress at each concentration. Here we provide the first evidence for the cytoprotective effect of short-term GPC treatment. However, long-term administration of GPC may exert cytotoxicity in a wide concentration range in cardiac myocytes. These results may draw attention to a comprehensive cardiac safety protocol for the testing of GPC.

Original languageEnglish
JournalMolecular and Cellular Biochemistry
DOIs
Publication statusPublished - Jan 1 2019

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Glycerylphosphorylcholine
Oxidative stress
Cardiac Myocytes
Rats
Cells
Cell death
Superoxides
Cell Survival
Oxidative Stress
Cytotoxicity
Cell Death
Assays
Dietary Supplements
Reperfusion
Testing
Ischemia
Staining and Labeling
Safety
Experiments

Keywords

  • l-Alpha-glycerylphosphorylcholine
  • Mitochondrial dysfunction
  • Neonatal rat cardiac myocytes
  • Oxidative stress

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

l-Alpha-glycerylphosphorylcholine can be cytoprotective or cytotoxic in neonatal rat cardiac myocytes : a double-edged sword phenomenon. / Tuboly, Eszter; Gáspár, Renáta; Ibor, Miguel Olias; Gömöri, Kamilla; Kiss, Bernadett; Strifler, Gerda; Hartmann, P.; Ferdinandy, Péter; Bartekova, Monika; Borós, M.; Görbe, A.

In: Molecular and Cellular Biochemistry, 01.01.2019.

Research output: Contribution to journalArticle

Tuboly, Eszter ; Gáspár, Renáta ; Ibor, Miguel Olias ; Gömöri, Kamilla ; Kiss, Bernadett ; Strifler, Gerda ; Hartmann, P. ; Ferdinandy, Péter ; Bartekova, Monika ; Borós, M. ; Görbe, A. / l-Alpha-glycerylphosphorylcholine can be cytoprotective or cytotoxic in neonatal rat cardiac myocytes : a double-edged sword phenomenon. In: Molecular and Cellular Biochemistry. 2019.
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AU - Tuboly, Eszter

AU - Gáspár, Renáta

AU - Ibor, Miguel Olias

AU - Gömöri, Kamilla

AU - Kiss, Bernadett

AU - Strifler, Gerda

AU - Hartmann, P.

AU - Ferdinandy, Péter

AU - Bartekova, Monika

AU - Borós, M.

AU - Görbe, A.

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N2 - l-Alpha-glycerylphosphorylcholine (GPC) is a widely used food supplement. GPC has been shown to exert beneficial effects in several organs; however, the cardiac effects of GPC have yet to be investigated. The aim of the present study was therefore to map out the effects of GPC on cardiac myocytes, with or without ischemia–reperfusion insult. Neonatal rat cardiac myocytes were treated with GPC at 1, 10, 80, and 100 µM concentrations for 15 min, 3 h, or 24 h, respectively. Cell viability by calcein assay and the degree of oxidative stress by DHE (superoxide level) and H2DCF (total ROS accumulation) staining were measured. In separate experiments, cardiomyocytes were pre-treated with the optimal concentration of GPC for 3 h and then cells were exposed to 4 h of simulated ischemia followed by 2 h of reperfusion (SI/R). Cell viability was measured at the end of the SI/R protocol. In normoxic conditions, the 15-min and the 3-h GPC treatment did not affect cell viability, total ROS, and superoxide levels. Under SI/R conditions, the 3-h GPC treatment protected the cardiac myocytes from SI/R-induced cell death and did not alter the level of oxidative stress. The 24-h GPC treatment in normoxic conditions resulted in significant cell death and increased oxidative stress at each concentration. Here we provide the first evidence for the cytoprotective effect of short-term GPC treatment. However, long-term administration of GPC may exert cytotoxicity in a wide concentration range in cardiac myocytes. These results may draw attention to a comprehensive cardiac safety protocol for the testing of GPC.

AB - l-Alpha-glycerylphosphorylcholine (GPC) is a widely used food supplement. GPC has been shown to exert beneficial effects in several organs; however, the cardiac effects of GPC have yet to be investigated. The aim of the present study was therefore to map out the effects of GPC on cardiac myocytes, with or without ischemia–reperfusion insult. Neonatal rat cardiac myocytes were treated with GPC at 1, 10, 80, and 100 µM concentrations for 15 min, 3 h, or 24 h, respectively. Cell viability by calcein assay and the degree of oxidative stress by DHE (superoxide level) and H2DCF (total ROS accumulation) staining were measured. In separate experiments, cardiomyocytes were pre-treated with the optimal concentration of GPC for 3 h and then cells were exposed to 4 h of simulated ischemia followed by 2 h of reperfusion (SI/R). Cell viability was measured at the end of the SI/R protocol. In normoxic conditions, the 15-min and the 3-h GPC treatment did not affect cell viability, total ROS, and superoxide levels. Under SI/R conditions, the 3-h GPC treatment protected the cardiac myocytes from SI/R-induced cell death and did not alter the level of oxidative stress. The 24-h GPC treatment in normoxic conditions resulted in significant cell death and increased oxidative stress at each concentration. Here we provide the first evidence for the cytoprotective effect of short-term GPC treatment. However, long-term administration of GPC may exert cytotoxicity in a wide concentration range in cardiac myocytes. These results may draw attention to a comprehensive cardiac safety protocol for the testing of GPC.

KW - l-Alpha-glycerylphosphorylcholine

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KW - Oxidative stress

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