L-alpha glycerylphosphorylcholine as a potential radioprotective agent in zebrafish embryo model

Emília Rita Szabó, Imola Plangár, Tünde Tokés, Imola Mán, Róbert Polanek, Róbert Kovács, Gábor Fekete, Zoltán Szabó, Zsolt Csenki, F. Baska, Katalin Hideghéty

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This work establishes the zebrafish embryo model for ionizing radiation (IR) modifier research and also evaluates the protective effect of l-alpha glycerylphosphorylcholine (GPC). Embryos were exposed to a single-fraction whole-body gamma irradiation (5, 10, 15, and 20 Gy) at different postfertilization time points and were serially assessed for viability and macro- and micromorphologic abnormalities. After toxicity evaluation, 194 μM of GPC was added for certain groups with 3-h incubation before the radiation. Nuclear factor kappa B (NF-κB) and interleukin-1β (IL-1β) expression changes were measured using quantitative real-time polymerase chain reaction. A higher sensitivity could be observed at earlier stages of the embryogenesis. The lethal dose (LD50) for 6 hours postfertilization (hpf) embryos was 15 Gy and for 24 hpf was 20 Gy on day 7, respectively. GPC administration resulted in a significant improvement in both the distortion rate and survival of the 24 hpf embryos. Qualitative evaluation of the histological changes confirmed the protective effect of GPC. IL-1β and NF-κB overexpression due to 10 Gy irradiation was also reduced by GPC. GPC exhibited promising radioprotective effects in our zebrafish embryo model, decreasing the irradiation-induced morphological damage and lethality with significant reduction of IR-caused pro-inflammatory activation.

Original languageEnglish
Pages (from-to)481-488
Number of pages8
JournalZebrafish
Volume13
Issue number6
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Glycerylphosphorylcholine
Radiation-Protective Agents
Zebrafish
Danio rerio
embryo (animal)
Embryonic Structures
interleukin-1
ionizing radiation
NF-kappa B
protective effect
Ionizing Radiation
Interleukin-1
irradiation
modifiers (genes)
lethal dose
lethal dose 50
Whole-Body Irradiation
Lethal Dose 50
gamma radiation
quantitative polymerase chain reaction

Keywords

  • L-alpha glycerylphosphorylcholine
  • NFκB
  • radiobiology
  • radioprotection
  • zebrafish

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology

Cite this

Szabó, E. R., Plangár, I., Tokés, T., Mán, I., Polanek, R., Kovács, R., ... Hideghéty, K. (2016). L-alpha glycerylphosphorylcholine as a potential radioprotective agent in zebrafish embryo model. Zebrafish, 13(6), 481-488. https://doi.org/10.1089/zeb.2016.1269

L-alpha glycerylphosphorylcholine as a potential radioprotective agent in zebrafish embryo model. / Szabó, Emília Rita; Plangár, Imola; Tokés, Tünde; Mán, Imola; Polanek, Róbert; Kovács, Róbert; Fekete, Gábor; Szabó, Zoltán; Csenki, Zsolt; Baska, F.; Hideghéty, Katalin.

In: Zebrafish, Vol. 13, No. 6, 01.01.2016, p. 481-488.

Research output: Contribution to journalArticle

Szabó, ER, Plangár, I, Tokés, T, Mán, I, Polanek, R, Kovács, R, Fekete, G, Szabó, Z, Csenki, Z, Baska, F & Hideghéty, K 2016, 'L-alpha glycerylphosphorylcholine as a potential radioprotective agent in zebrafish embryo model', Zebrafish, vol. 13, no. 6, pp. 481-488. https://doi.org/10.1089/zeb.2016.1269
Szabó ER, Plangár I, Tokés T, Mán I, Polanek R, Kovács R et al. L-alpha glycerylphosphorylcholine as a potential radioprotective agent in zebrafish embryo model. Zebrafish. 2016 Jan 1;13(6):481-488. https://doi.org/10.1089/zeb.2016.1269
Szabó, Emília Rita ; Plangár, Imola ; Tokés, Tünde ; Mán, Imola ; Polanek, Róbert ; Kovács, Róbert ; Fekete, Gábor ; Szabó, Zoltán ; Csenki, Zsolt ; Baska, F. ; Hideghéty, Katalin. / L-alpha glycerylphosphorylcholine as a potential radioprotective agent in zebrafish embryo model. In: Zebrafish. 2016 ; Vol. 13, No. 6. pp. 481-488.
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