Kringle 4 of human apolipoprotein[a] shares a linear antigenic site with human catalase

H. Dieplinger, G. Gruber, K. Krasznai, S. Reschauer, C. Seidel, G. Burns, H. J. Muller, A. Császár, W. Vogel, H. Robenek, G. Utermann

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Abstract

Monoclonal antibody (mab) 1A2, directed against human apolipoprotein[a] (apo[a]), revealed a strong reaction with peroxisomes as shown by immuno- gold labeled cryosections of human liver biopsies. This reactivity was not due to the presence of apo[al in peroxisomes but to a cross-reactivity of mab 1A2. Immunoblot analysis of peroxisomal fractions and purified human catalase demonstrated that mab 1A2 reacts with catalase. Conversely, an anti-catalase antibody also recognized apo[a]. By sequence comparison we identified a 4- amino acid motif(Y-Y-P-N) that is shared between the highly repetitive kringle 4 motif of apo[a] and the carboxy-terminal third of the peroxisomal marker enzyme catalase. No other identical sequences were identified in these proteins. Results from the following experiments indicated that 1A2 recognizes this short linear epitope. i) Mab 1A2 reacted only with the 4 amino acid peptide sequence in a pin-ELISA using immobilized overlapping peptides. ii) A synthetic peptide including this sequence completely inhibited the 1A2 immunoreactivity to apo[a] and catalase. iii) A recombinant fusion protein tagged with the putative epitope was recognized by mab 1A2. Our findings demonstrate that unknown linear epitopes in native proteins can be identified by sequence comparison between known proteins. The practical implication is that antibodies against apo[a] must be controlled for this cross-reactivity before using them for immunohistochemical studies of intracellular apo[a] in tissues or cells.

Original languageEnglish
Pages (from-to)813-822
Number of pages10
JournalJournal of Lipid Research
Volume36
Issue number4
Publication statusPublished - 1995

Fingerprint

Kringles
Apolipoproteins
Catalase
Monoclonal Antibodies
Epitopes
Peroxisomes
Peptides
Recombinant Fusion Proteins
Amino Acids
Amino Acid Motifs
Proteins
Antibodies
Biopsy
Gold
Liver
Anti-Idiotypic Antibodies
Amino Acid Sequence
Enzyme-Linked Immunosorbent Assay
Tissue
Enzymes

Keywords

  • cross-reaction
  • epitope
  • monoclonal antibody
  • sequence alignment

ASJC Scopus subject areas

  • Endocrinology

Cite this

Dieplinger, H., Gruber, G., Krasznai, K., Reschauer, S., Seidel, C., Burns, G., ... Utermann, G. (1995). Kringle 4 of human apolipoprotein[a] shares a linear antigenic site with human catalase. Journal of Lipid Research, 36(4), 813-822.

Kringle 4 of human apolipoprotein[a] shares a linear antigenic site with human catalase. / Dieplinger, H.; Gruber, G.; Krasznai, K.; Reschauer, S.; Seidel, C.; Burns, G.; Muller, H. J.; Császár, A.; Vogel, W.; Robenek, H.; Utermann, G.

In: Journal of Lipid Research, Vol. 36, No. 4, 1995, p. 813-822.

Research output: Contribution to journalArticle

Dieplinger, H, Gruber, G, Krasznai, K, Reschauer, S, Seidel, C, Burns, G, Muller, HJ, Császár, A, Vogel, W, Robenek, H & Utermann, G 1995, 'Kringle 4 of human apolipoprotein[a] shares a linear antigenic site with human catalase', Journal of Lipid Research, vol. 36, no. 4, pp. 813-822.
Dieplinger H, Gruber G, Krasznai K, Reschauer S, Seidel C, Burns G et al. Kringle 4 of human apolipoprotein[a] shares a linear antigenic site with human catalase. Journal of Lipid Research. 1995;36(4):813-822.
Dieplinger, H. ; Gruber, G. ; Krasznai, K. ; Reschauer, S. ; Seidel, C. ; Burns, G. ; Muller, H. J. ; Császár, A. ; Vogel, W. ; Robenek, H. ; Utermann, G. / Kringle 4 of human apolipoprotein[a] shares a linear antigenic site with human catalase. In: Journal of Lipid Research. 1995 ; Vol. 36, No. 4. pp. 813-822.
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abstract = "Monoclonal antibody (mab) 1A2, directed against human apolipoprotein[a] (apo[a]), revealed a strong reaction with peroxisomes as shown by immuno- gold labeled cryosections of human liver biopsies. This reactivity was not due to the presence of apo[al in peroxisomes but to a cross-reactivity of mab 1A2. Immunoblot analysis of peroxisomal fractions and purified human catalase demonstrated that mab 1A2 reacts with catalase. Conversely, an anti-catalase antibody also recognized apo[a]. By sequence comparison we identified a 4- amino acid motif(Y-Y-P-N) that is shared between the highly repetitive kringle 4 motif of apo[a] and the carboxy-terminal third of the peroxisomal marker enzyme catalase. No other identical sequences were identified in these proteins. Results from the following experiments indicated that 1A2 recognizes this short linear epitope. i) Mab 1A2 reacted only with the 4 amino acid peptide sequence in a pin-ELISA using immobilized overlapping peptides. ii) A synthetic peptide including this sequence completely inhibited the 1A2 immunoreactivity to apo[a] and catalase. iii) A recombinant fusion protein tagged with the putative epitope was recognized by mab 1A2. Our findings demonstrate that unknown linear epitopes in native proteins can be identified by sequence comparison between known proteins. The practical implication is that antibodies against apo[a] must be controlled for this cross-reactivity before using them for immunohistochemical studies of intracellular apo[a] in tissues or cells.",
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AU - Gruber, G.

AU - Krasznai, K.

AU - Reschauer, S.

AU - Seidel, C.

AU - Burns, G.

AU - Muller, H. J.

AU - Császár, A.

AU - Vogel, W.

AU - Robenek, H.

AU - Utermann, G.

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