Kocuria palustris sp. nov. and Kocuria rhizophila sp. nov., isolated from the rhizoplane of the narrow-leaved cattail (Typha angustifolia)

G. Kovács, Jutta Burghardt, Silke Pradella, Peter Schumann, Erko Stackebrandt, K. Márialigeti

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Two Gram-positive, aerobic spherical actinobacteria were isolated from the rhizoplane of narrow-leaved cattail (Typha angustifolia) collected from a floating mat in the Soroksar tributary of the Danube river, Hungary. Sequence comparisons of the 16S rDNA indicated these isolates to be phylogenetic neighbours of members of the genus Kocuria, family Micrococcaceae, in which they represent two novel lineages. The phylogenetic distinctness of the two organisms TA68(T) and TAGA27(T) was supported by DNA-DNA similarity values of less than 55% between each other and with the type strains of Kocuria rosea, Kocuria kristinae and Kocuria varians. Chemotaxonomic properties supported the placement of the two isolates in the genus Kocuria. The diagnostic diamino acid of the cell-wall peptidoglycan is lysine, the interpeptide bridge is composed of three alanine residues. Predominant menaquinone was MK- 7(H2). The fatty acid pattern represents the straight-chain saturated iso- anteiso type. Main fatty acid was anteiso-C(15:0). The phospholipids are diphosphatidylglycerol, phosphatidylglycerol and an unknown component. The DNA base composition of strains TA68(T) and TAGA27(T) is 69.4 and 69.6 mol% G+C, respectively. Genotypic, morphological and physiological characteristics are used to describe two new species of Kocuria, for which we propose the names Kocuria palustris, type strain DSM 11925(T) and Kocuria rhizophila, type strain DSM 11926(T).

Original languageEnglish
Pages (from-to)167-173
Number of pages7
JournalInternational Journal of Systematic Bacteriology
Volume49
Issue number1
Publication statusPublished - Jan 1999

Fingerprint

Kocuria palustris
Kocuria rhizophila
Typhaceae
Typha angustifolia
rhizoplane
Kocuria
DNA
new species
Micrococcaceae
Fatty Acids
fatty acid
phylogenetics
Vitamin K 2
Kocuria kristinae
Phosphatidylglycerols
Cardiolipins
Kocuria rosea
Peptidoglycan
Hungary
Kocuria varians

Keywords

  • Classification
  • Kocuria palustris
  • Kocuria rhizophila
  • Phylogeny

ASJC Scopus subject areas

  • Microbiology
  • Immunology

Cite this

Kocuria palustris sp. nov. and Kocuria rhizophila sp. nov., isolated from the rhizoplane of the narrow-leaved cattail (Typha angustifolia). / Kovács, G.; Burghardt, Jutta; Pradella, Silke; Schumann, Peter; Stackebrandt, Erko; Márialigeti, K.

In: International Journal of Systematic Bacteriology, Vol. 49, No. 1, 01.1999, p. 167-173.

Research output: Contribution to journalArticle

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abstract = "Two Gram-positive, aerobic spherical actinobacteria were isolated from the rhizoplane of narrow-leaved cattail (Typha angustifolia) collected from a floating mat in the Soroksar tributary of the Danube river, Hungary. Sequence comparisons of the 16S rDNA indicated these isolates to be phylogenetic neighbours of members of the genus Kocuria, family Micrococcaceae, in which they represent two novel lineages. The phylogenetic distinctness of the two organisms TA68(T) and TAGA27(T) was supported by DNA-DNA similarity values of less than 55{\%} between each other and with the type strains of Kocuria rosea, Kocuria kristinae and Kocuria varians. Chemotaxonomic properties supported the placement of the two isolates in the genus Kocuria. The diagnostic diamino acid of the cell-wall peptidoglycan is lysine, the interpeptide bridge is composed of three alanine residues. Predominant menaquinone was MK- 7(H2). The fatty acid pattern represents the straight-chain saturated iso- anteiso type. Main fatty acid was anteiso-C(15:0). The phospholipids are diphosphatidylglycerol, phosphatidylglycerol and an unknown component. The DNA base composition of strains TA68(T) and TAGA27(T) is 69.4 and 69.6 mol{\%} G+C, respectively. Genotypic, morphological and physiological characteristics are used to describe two new species of Kocuria, for which we propose the names Kocuria palustris, type strain DSM 11925(T) and Kocuria rhizophila, type strain DSM 11926(T).",
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