Knockdown of the α5 laminin chain affects differentiation of colorectal cancer cells and their sensitivity to chemotherapy

Diana Maltseva, Maria Raygorodskaya, Evgeny Knyazev, Victor Zgoda, Olga Tikhonova, Shan Zaidi, Sergey Nikulin, Ancha Baranova, Andrey Turchinovich, Sergey Rodin, Alexander Tonevitsky

Research output: Contribution to journalArticle

Abstract

The interaction of tumor cells with the extracellular matrix (ECM) may affect the rate of cancer progression and metastasis. One of the major components of ECM are laminins, the heterotrimeric glycoproteins consisting of α-, β-, and γ-chains (αβγ). Laminins interact with their cell surface receptors and, thus, regulate multiple cellular processes. In this work, we demonstrate that shRNA-mediated knockdown of the α5 laminin chain results in Wnt- and mTORC1-dependent partial dedifferentiation of colorectal cancer cells. Furthermore, we showed that this dedifferentiation involved activation of ER-stress signaling, pathway promoting the sensitivity of cells to 5-fluorouracil.

Original languageEnglish
Pages (from-to)107-116
Number of pages10
JournalBiochimie
Volume174
DOIs
Publication statusPublished - Jul 2020

Keywords

  • Colorectal cancer
  • Dedifferentiation
  • HT29
  • Intestinal epithelium stem cell marker
  • LAMA5
  • Laminin

ASJC Scopus subject areas

  • Biochemistry

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  • Cite this

    Maltseva, D., Raygorodskaya, M., Knyazev, E., Zgoda, V., Tikhonova, O., Zaidi, S., Nikulin, S., Baranova, A., Turchinovich, A., Rodin, S., & Tonevitsky, A. (2020). Knockdown of the α5 laminin chain affects differentiation of colorectal cancer cells and their sensitivity to chemotherapy. Biochimie, 174, 107-116. https://doi.org/10.1016/j.biochi.2020.04.016