Kinolonrezisztencia-plazmidok molekuláris epidemiológiai jellemzése sertés eredetu, multirezisztens, kommenzalista E. coli törzsekben

Szmolka Ama, Daniela Fortinp, Laura Villa, Alessandra Carattoli, Muna F. Anjum, B. Nagy

Research output: Contribution to journalArticle

Abstract

The authors briefly discuss the quinolone resistance in Gram-negative bacteria, and give information about the main characteristics and genes of plasmid-mediated quinolone resistance (PMQR). They briefly review the molecular epidemiology methods: PCR microarray technology, the multilocus sequence typing (MLST) and the PCR-based plasmid replicon typing (PBRT), which they used, together with other known methods (PCR, plasmid analysis, and cloning/sequencing) in these studies. The authors aimed to report the results of their earlier studies of Szmolka et al. (38) on PMQR of porcine E. coli in two large piggeries in Hungary and Romania. The study identified PMQR E. coli strains in 34% of piglets in the Romanian pig farm. Clonality of six qnrS1 E. coli strains representing that farm was established by MLST, and the qnrS1 plasmids were characterized by plasmid transfer and by PBRT. Microarray studies were performed using AMR05 and Ec03 PCR-microarray systems for the detection of -130 antimicrobial resistance and virulence genes of E. coli. As a result, the six tested qnrS1 strains were assigned to three different MLST types. PCR-based replicon typing proved that they all carry IncN plasmids, representing the first IncN-borne qnrS1 gene identified in E. coli from food producing animals. There were no virulence genes associated with IncN type qnrS1 plasmids, but they were responsible for the co-transfer of b/aTEM-1 and tet(A) genes responsible for ampicillin and tetracycline resistance. The genetic environment of the qnrS1 gene showed ≥99% homology with the corresponding resistance region of the plNF5 plasmid from Salmonella Infantis isolated from chicken carcass and of IncN plasmids isolated from human clinical E. coli strains. Thus, the data suggests that transfer of qnrS1 plasmids occurs between Salmonella and E. coli of animal and human origin with pigs representing one of the potential reservoirs.

Original languageHungarian
Pages (from-to)297-308
Number of pages12
JournalMagyar Allatorvosok Lapja
Volume134
Issue number5
Publication statusPublished - May 2012

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Kinolonrezisztencia-plazmidok molekuláris epidemiológiai jellemzése sertés eredetu, multirezisztens, kommenzalista E. coli törzsekben. / Ama, Szmolka; Fortinp, Daniela; Villa, Laura; Carattoli, Alessandra; Anjum, Muna F.; Nagy, B.

In: Magyar Allatorvosok Lapja, Vol. 134, No. 5, 05.2012, p. 297-308.

Research output: Contribution to journalArticle

Ama, Szmolka ; Fortinp, Daniela ; Villa, Laura ; Carattoli, Alessandra ; Anjum, Muna F. ; Nagy, B. / Kinolonrezisztencia-plazmidok molekuláris epidemiológiai jellemzése sertés eredetu, multirezisztens, kommenzalista E. coli törzsekben. In: Magyar Allatorvosok Lapja. 2012 ; Vol. 134, No. 5. pp. 297-308.
@article{62cf6a78d3f34ec499526cced5cdce22,
title = "Kinolonrezisztencia-plazmidok molekul{\'a}ris epidemiol{\'o}giai jellemz{\'e}se sert{\'e}s eredetu, multirezisztens, kommenzalista E. coli t{\"o}rzsekben",
abstract = "The authors briefly discuss the quinolone resistance in Gram-negative bacteria, and give information about the main characteristics and genes of plasmid-mediated quinolone resistance (PMQR). They briefly review the molecular epidemiology methods: PCR microarray technology, the multilocus sequence typing (MLST) and the PCR-based plasmid replicon typing (PBRT), which they used, together with other known methods (PCR, plasmid analysis, and cloning/sequencing) in these studies. The authors aimed to report the results of their earlier studies of Szmolka et al. (38) on PMQR of porcine E. coli in two large piggeries in Hungary and Romania. The study identified PMQR E. coli strains in 34{\%} of piglets in the Romanian pig farm. Clonality of six qnrS1 E. coli strains representing that farm was established by MLST, and the qnrS1 plasmids were characterized by plasmid transfer and by PBRT. Microarray studies were performed using AMR05 and Ec03 PCR-microarray systems for the detection of -130 antimicrobial resistance and virulence genes of E. coli. As a result, the six tested qnrS1 strains were assigned to three different MLST types. PCR-based replicon typing proved that they all carry IncN plasmids, representing the first IncN-borne qnrS1 gene identified in E. coli from food producing animals. There were no virulence genes associated with IncN type qnrS1 plasmids, but they were responsible for the co-transfer of b/aTEM-1 and tet(A) genes responsible for ampicillin and tetracycline resistance. The genetic environment of the qnrS1 gene showed ≥99{\%} homology with the corresponding resistance region of the plNF5 plasmid from Salmonella Infantis isolated from chicken carcass and of IncN plasmids isolated from human clinical E. coli strains. Thus, the data suggests that transfer of qnrS1 plasmids occurs between Salmonella and E. coli of animal and human origin with pigs representing one of the potential reservoirs.",
author = "Szmolka Ama and Daniela Fortinp and Laura Villa and Alessandra Carattoli and Anjum, {Muna F.} and B. Nagy",
year = "2012",
month = "5",
language = "Hungarian",
volume = "134",
pages = "297--308",
journal = "Magyar Allatorvosok Lapja",
issn = "0025-004X",
publisher = "Magyar Mezogazdasag Ltd",
number = "5",

}

TY - JOUR

T1 - Kinolonrezisztencia-plazmidok molekuláris epidemiológiai jellemzése sertés eredetu, multirezisztens, kommenzalista E. coli törzsekben

AU - Ama, Szmolka

AU - Fortinp, Daniela

AU - Villa, Laura

AU - Carattoli, Alessandra

AU - Anjum, Muna F.

AU - Nagy, B.

PY - 2012/5

Y1 - 2012/5

N2 - The authors briefly discuss the quinolone resistance in Gram-negative bacteria, and give information about the main characteristics and genes of plasmid-mediated quinolone resistance (PMQR). They briefly review the molecular epidemiology methods: PCR microarray technology, the multilocus sequence typing (MLST) and the PCR-based plasmid replicon typing (PBRT), which they used, together with other known methods (PCR, plasmid analysis, and cloning/sequencing) in these studies. The authors aimed to report the results of their earlier studies of Szmolka et al. (38) on PMQR of porcine E. coli in two large piggeries in Hungary and Romania. The study identified PMQR E. coli strains in 34% of piglets in the Romanian pig farm. Clonality of six qnrS1 E. coli strains representing that farm was established by MLST, and the qnrS1 plasmids were characterized by plasmid transfer and by PBRT. Microarray studies were performed using AMR05 and Ec03 PCR-microarray systems for the detection of -130 antimicrobial resistance and virulence genes of E. coli. As a result, the six tested qnrS1 strains were assigned to three different MLST types. PCR-based replicon typing proved that they all carry IncN plasmids, representing the first IncN-borne qnrS1 gene identified in E. coli from food producing animals. There were no virulence genes associated with IncN type qnrS1 plasmids, but they were responsible for the co-transfer of b/aTEM-1 and tet(A) genes responsible for ampicillin and tetracycline resistance. The genetic environment of the qnrS1 gene showed ≥99% homology with the corresponding resistance region of the plNF5 plasmid from Salmonella Infantis isolated from chicken carcass and of IncN plasmids isolated from human clinical E. coli strains. Thus, the data suggests that transfer of qnrS1 plasmids occurs between Salmonella and E. coli of animal and human origin with pigs representing one of the potential reservoirs.

AB - The authors briefly discuss the quinolone resistance in Gram-negative bacteria, and give information about the main characteristics and genes of plasmid-mediated quinolone resistance (PMQR). They briefly review the molecular epidemiology methods: PCR microarray technology, the multilocus sequence typing (MLST) and the PCR-based plasmid replicon typing (PBRT), which they used, together with other known methods (PCR, plasmid analysis, and cloning/sequencing) in these studies. The authors aimed to report the results of their earlier studies of Szmolka et al. (38) on PMQR of porcine E. coli in two large piggeries in Hungary and Romania. The study identified PMQR E. coli strains in 34% of piglets in the Romanian pig farm. Clonality of six qnrS1 E. coli strains representing that farm was established by MLST, and the qnrS1 plasmids were characterized by plasmid transfer and by PBRT. Microarray studies were performed using AMR05 and Ec03 PCR-microarray systems for the detection of -130 antimicrobial resistance and virulence genes of E. coli. As a result, the six tested qnrS1 strains were assigned to three different MLST types. PCR-based replicon typing proved that they all carry IncN plasmids, representing the first IncN-borne qnrS1 gene identified in E. coli from food producing animals. There were no virulence genes associated with IncN type qnrS1 plasmids, but they were responsible for the co-transfer of b/aTEM-1 and tet(A) genes responsible for ampicillin and tetracycline resistance. The genetic environment of the qnrS1 gene showed ≥99% homology with the corresponding resistance region of the plNF5 plasmid from Salmonella Infantis isolated from chicken carcass and of IncN plasmids isolated from human clinical E. coli strains. Thus, the data suggests that transfer of qnrS1 plasmids occurs between Salmonella and E. coli of animal and human origin with pigs representing one of the potential reservoirs.

UR - http://www.scopus.com/inward/record.url?scp=84861882308&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861882308&partnerID=8YFLogxK

M3 - Article

VL - 134

SP - 297

EP - 308

JO - Magyar Allatorvosok Lapja

JF - Magyar Allatorvosok Lapja

SN - 0025-004X

IS - 5

ER -