Kinetics of the flash-induced electrochromic absorbance change in the presence of background illumination. Turnover rate of the electron transport. I. Isolated intact chloroplasts

Klára Barabás, L. Zimányi, G. Garab

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We investigated the flash-induced electrochromic absorbance change, ΔA515, of isolated intact chloroplasts in continuous monochromatic background light of different intensities and wavelengths. From the variation of the amplitude of ΔA515 in background illumination the steady-state turnover time of electron transport was found to be around 100 msec and the slowest process could be assigned to a photosystem 1 driven cycle. The change of ΔpH induced by nigericin, ATP, or ADP did not modify substantially the turnover time. In contrast to earlier observations the slow rise (∼10 msec) of ΔA515 in untreated chloroplasts persists also at high-intensity background illumination exciting both photosystems. The proportion of the slow rise of ΔA515 in nigericin-treated chloroplasts increases in the presence of background light. This result on the slow rise is discussed in terms of two different models existing in the literature.

Original languageEnglish
Pages (from-to)349-364
Number of pages16
JournalJournal of Bioenergetics and Biomembranes
Volume17
Issue number6
DOIs
Publication statusPublished - Dec 1985

Fingerprint

Chloroplasts
Electron Transport
Lighting
Nigericin
Light
Adenosine Diphosphate
Adenosine Triphosphate

Keywords

  • electrochromism
  • Intact chloroplasts
  • slow rise of ΔA
  • turnover rate of electron transport

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

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title = "Kinetics of the flash-induced electrochromic absorbance change in the presence of background illumination. Turnover rate of the electron transport. I. Isolated intact chloroplasts",
abstract = "We investigated the flash-induced electrochromic absorbance change, ΔA515, of isolated intact chloroplasts in continuous monochromatic background light of different intensities and wavelengths. From the variation of the amplitude of ΔA515 in background illumination the steady-state turnover time of electron transport was found to be around 100 msec and the slowest process could be assigned to a photosystem 1 driven cycle. The change of ΔpH induced by nigericin, ATP, or ADP did not modify substantially the turnover time. In contrast to earlier observations the slow rise (∼10 msec) of ΔA515 in untreated chloroplasts persists also at high-intensity background illumination exciting both photosystems. The proportion of the slow rise of ΔA515 in nigericin-treated chloroplasts increases in the presence of background light. This result on the slow rise is discussed in terms of two different models existing in the literature.",
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author = "Kl{\'a}ra Barab{\'a}s and L. Zim{\'a}nyi and G. Garab",
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T1 - Kinetics of the flash-induced electrochromic absorbance change in the presence of background illumination. Turnover rate of the electron transport. I. Isolated intact chloroplasts

AU - Barabás, Klára

AU - Zimányi, L.

AU - Garab, G.

PY - 1985/12

Y1 - 1985/12

N2 - We investigated the flash-induced electrochromic absorbance change, ΔA515, of isolated intact chloroplasts in continuous monochromatic background light of different intensities and wavelengths. From the variation of the amplitude of ΔA515 in background illumination the steady-state turnover time of electron transport was found to be around 100 msec and the slowest process could be assigned to a photosystem 1 driven cycle. The change of ΔpH induced by nigericin, ATP, or ADP did not modify substantially the turnover time. In contrast to earlier observations the slow rise (∼10 msec) of ΔA515 in untreated chloroplasts persists also at high-intensity background illumination exciting both photosystems. The proportion of the slow rise of ΔA515 in nigericin-treated chloroplasts increases in the presence of background light. This result on the slow rise is discussed in terms of two different models existing in the literature.

AB - We investigated the flash-induced electrochromic absorbance change, ΔA515, of isolated intact chloroplasts in continuous monochromatic background light of different intensities and wavelengths. From the variation of the amplitude of ΔA515 in background illumination the steady-state turnover time of electron transport was found to be around 100 msec and the slowest process could be assigned to a photosystem 1 driven cycle. The change of ΔpH induced by nigericin, ATP, or ADP did not modify substantially the turnover time. In contrast to earlier observations the slow rise (∼10 msec) of ΔA515 in untreated chloroplasts persists also at high-intensity background illumination exciting both photosystems. The proportion of the slow rise of ΔA515 in nigericin-treated chloroplasts increases in the presence of background light. This result on the slow rise is discussed in terms of two different models existing in the literature.

KW - electrochromism

KW - Intact chloroplasts

KW - slow rise of ΔA

KW - turnover rate of electron transport

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