Kinetics of Coupled Reactions Catalyzed by Aspartate Aminotransferase and Glutamate Dehydrogenase

Costantino SALERNO, Judit OVÁDI, Tamás KELETI, Paolo FASELLA

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Abstract

Liver mitochondrial aspartate aminotransferase and glutamate dehydrogenase catalyze the following sequence of reactions: (Formula Presented.) In the presence of a slight excess of dehydrogenase, the time course of NADPH oxidation resulting from the overall reaction goes through a lag phase and reaches a linear phase. The slope of the linear part of this curve is a linear function of transaminase concentration. At high concentration (∼ 10 μM) of both enzymes the lag phase, as observed after rapid mixing of the two enzymes in a Durrum stopped‐flow spectrophotometer, is shorter than that predicted from the kinetic parameters determined for the separate reactions catalyzed by each enzyme

Original languageEnglish
Pages (from-to)511-517
Number of pages7
JournalEuropean Journal of Biochemistry
Volume121
Issue number3
DOIs
Publication statusPublished - Jan 1982

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ASJC Scopus subject areas

  • Biochemistry

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