Kinetic studies of citrate synthase from rat kidney and rat brain

Y. Matsuoka, P. Srere

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Crystalline citrate synthase (citrate oxalacetate lyase (coenzyme A acetylating) EC 4.1.3.7) has been prepared from rat kidney and rat brain. These enzymes are homogeneous as judged by acrylamide gel electrophoresis, and immunologically identical with the rat heart citrate synthase. Steady state kinetic studies including product inhibition experiments indicate that the substrates are added in a random order, the products come off in random order, and that two dead end complexes are formed. When acetyl CoA concentrations are varied over wide range, a nonlinear Lineweaver Burk plot is obtained suggesting apparant substrate activation. One interpretation of this observation is that some cooperativity exists between two sites on the enzyme.

Original languageEnglish
Pages (from-to)8022-8030
Number of pages9
JournalJournal of Biological Chemistry
Volume248
Issue number23
Publication statusPublished - 1973

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Citrate (si)-Synthase
Rats
Brain
citrate (pro-3S)-lyase
Kidney
Kinetics
Acetyl Coenzyme A
Lyases
Acrylamide
Substrates
Enzymes
Coenzyme A
Electrophoresis
Citric Acid
Gels
Chemical activation
Crystalline materials
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kinetic studies of citrate synthase from rat kidney and rat brain. / Matsuoka, Y.; Srere, P.

In: Journal of Biological Chemistry, Vol. 248, No. 23, 1973, p. 8022-8030.

Research output: Contribution to journalArticle

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