Kinetic resolutions with novel, highly enantioselective fungal lipases produced by solid state fermentation

Viviána Nagy, Eniko R. Toke, Lee Chee Keong, Gábor Szatzker, Darah Ibrahim, Ibrahim Che Omar, G. Szakács, L. Poppe

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Thirty-eight filamentous fungi cultivated under solid state fermentation (SSF) conditions were screened for lipase activity and enantioselectivity in kinetic resolutions of racemic secondary alcohols (rac-1a-c) by acetylation with vinyl acetate performed in organic solvents. Many of the target fungi have not been studied previously for lipase/esterase activity and enantioselectivity. Without special enzyme isolation processes, the room temperature (25 °C) dried SSF cultures as such were tested in the enantiomer selective biotransformations. The majority of these SSF preparations proved to be effective as enantiomer selective biocatalysts exhibiting high but usual enantioselectivities according to the Kazlauskas rule. However, the SSF preparation of Mucor hiemalis origin acted as a selective anti-Kazlauskas catalyst. The best SSF products were successfully applied in preparative scale resolutions.

Original languageEnglish
Pages (from-to)141-148
Number of pages8
JournalJournal of Molecular Catalysis B: Enzymatic
Volume39
Issue number1-4
DOIs
Publication statusPublished - May 2 2006

Fingerprint

Enzyme kinetics
Lipases
Lipase
Fermentation
Enantioselectivity
Kinetics
Enantiomers
Fungi
Mucor
Acetylation
Biocatalysts
Esterases
Enzymes
Biotransformation
Organic solvents
Alcohols
Catalysts
Temperature

Keywords

  • Enantioselectivity
  • Kinetic resolution
  • Lipases
  • Solid state fermentation

ASJC Scopus subject areas

  • Biochemistry
  • Catalysis
  • Process Chemistry and Technology

Cite this

Kinetic resolutions with novel, highly enantioselective fungal lipases produced by solid state fermentation. / Nagy, Viviána; Toke, Eniko R.; Keong, Lee Chee; Szatzker, Gábor; Ibrahim, Darah; Omar, Ibrahim Che; Szakács, G.; Poppe, L.

In: Journal of Molecular Catalysis B: Enzymatic, Vol. 39, No. 1-4, 02.05.2006, p. 141-148.

Research output: Contribution to journalArticle

Nagy, Viviána ; Toke, Eniko R. ; Keong, Lee Chee ; Szatzker, Gábor ; Ibrahim, Darah ; Omar, Ibrahim Che ; Szakács, G. ; Poppe, L. / Kinetic resolutions with novel, highly enantioselective fungal lipases produced by solid state fermentation. In: Journal of Molecular Catalysis B: Enzymatic. 2006 ; Vol. 39, No. 1-4. pp. 141-148.
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