Kinetic assay for the determination of the oxidative stress biomarker, advanced oxidation protein products (AOPP) in the human blood plasma

L. Selmeci, L. Seres, P. Soós, M. Székely, G. Acsády

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A number of human diseases and pathological conditions were found to be associated with increased oxidative stress. In the literature several techniques are available for the assessment of oxidative stress, but most of them are not applicable for a routine medical laboratory due to the complex methodology and/or financial reasons. We report here on a simple, inexpensive, kinetic assay for the determination of the oxidative stress biomarker, advanced oxidation protein products (AOPP) in the human blood plasma. Methods: This study involved 70 patients (47M/23F; mean age: 64.6 y; range: 16-85) admitted to our Department with a wide range of cardiovascular and peripheral vascular diseases. Three critically ill patients were assigned for monitoring purposes. Plasma AOPP were simultaneously determined using an end-point assay as reference method and by a kinetic method developed in our laboratory. Plasma fibrinogen concentration was measured according to the Clauss method. Results: There was a highly significant correlation (r2=0.588; p2=0.780; p2=0.564; p

Original languageEnglish
Pages (from-to)209-218
Number of pages10
JournalActa Physiologica Hungarica
Volume95
Issue number2
DOIs
Publication statusPublished - Jun 2008

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Advanced Oxidation Protein Products
Oxidative Stress
Biomarkers
Endpoint Determination
Peripheral Vascular Diseases
Critical Illness
Fibrinogen

Keywords

  • Advanced oxidation protein products (AOPP)
  • Critically ill patients
  • Kinetic assay
  • Oxidative stress
  • Patient monitoring

ASJC Scopus subject areas

  • Physiology

Cite this

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AU - Seres, L.

AU - Soós, P.

AU - Székely, M.

AU - Acsády, G.

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