Kinetic and spectral resolution of multiple nonphotochemical quenching components in Arabidopsis leaves

P. Lambrev, Manuela Nilkens, Y. Miloslavina, Peter Jahns, Alfred R. Holzwarth

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Using novel specially designed instrumentation, fluorescence emission spectra were recorded from Arabidopsis (Arabidopsis thaliana) leaves during the induction period of dark to highlight adaptation in order to follow the spectral changes associated with the formation of nonphotochemical quenching. In addition to an overall decrease of photosystem II fluorescence (quenching) across the entire spectrum, high light induced two specific relative changes in the spectra: (1) a decrease of the main emission band at 682 nm relative to the farred (750-760 nm) part of the spectrum (ΔF682); and (2) an increase at 720 to 730 nm (ΔF720) relative to 750 to 760 nm. The kinetics of the two relative spectral changes and their dependence on various mutants revealed that they do not originate from the same process but rather from at least two independent processes. The ΔF720 change is specifically associated with the rapidly reversible energy-dependent quenching. Comparison of the wild-type Arabidopsis with mutants unable to produce or overexpressing the PsbS subunit of photosystem II showed that PsbS was a necessary component for ΔF720. The spectral change ΔF682 is induced both by energy-dependent quenching and by PsbS-independent mechanism(s). A third novel quenching process, independent from both PsbS and zeaxanthin, is activated by a high turnover rate of photosystem II. Its induction and relaxation occur on a time scale of a few minutes. Analysis of the spectral inhomogeneity of nonphotochemical quenching allows extraction of mechanistically valuable information from the fluorescence induction kinetics when registered in a spectrally resolved fashion.

Original languageEnglish
Pages (from-to)1611-1624
Number of pages14
JournalPlant Physiology
Volume152
Issue number3
DOIs
Publication statusPublished - Mar 2010

Fingerprint

Photosystem II Protein Complex
Arabidopsis
photosystem II
Fluorescence
fluorescence
kinetics
leaves
mutants
energy
zeaxanthin
instrumentation
scotophase
spectral analysis
Arabidopsis thaliana
Light

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Kinetic and spectral resolution of multiple nonphotochemical quenching components in Arabidopsis leaves. / Lambrev, P.; Nilkens, Manuela; Miloslavina, Y.; Jahns, Peter; Holzwarth, Alfred R.

In: Plant Physiology, Vol. 152, No. 3, 03.2010, p. 1611-1624.

Research output: Contribution to journalArticle

Lambrev, P. ; Nilkens, Manuela ; Miloslavina, Y. ; Jahns, Peter ; Holzwarth, Alfred R. / Kinetic and spectral resolution of multiple nonphotochemical quenching components in Arabidopsis leaves. In: Plant Physiology. 2010 ; Vol. 152, No. 3. pp. 1611-1624.
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