Kinetic analysis of the light-induced fluorescence quenching in light-harvesting chlorophyll a/b pigment-protein complex of photosystem II

Virginijus Barzda, Robert C. Jennings, Giuseppe Zucchelli, Gyözö Garab

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Abstract

We carried out a kinetic analysis of the light-induced fluorescence quenching (ΔF) of the light-harvesting chlorophyll a/b pigment-protein complex of photosystem II (LHCII) that was first observed by Jennings et al. (Photosynth. Res. 27, 57-64, 1991). We show that during a 2 min light, 2 min dark cycle, both the light and dark phases exhibit biexponential kinetics; this is tentatively explained by the presence of two types of light-induced quenchers in different domains of aggregated LHCII. Quantitative analysis could be carried out on the faster kinetic component; the slower component that was not completed during the measurement was not amenable for quantitative analysis. Our analysis revealed that the rate of the light-induced decrease of the fluorescence yield depended linearly on the light intensity, which shows that the generation of the quencher originates from a reaction that is first order with respect to the concentration of the excited domains. As shown by the estimated rate constant, photogeneration of the quencher is a fast reaction that can compete with other excitation-relaxation pathways. Both the decay and the recovery time constants of ΔF depended strongly on the temperature. Thermodynamic analysis showed that the fast light-induced decline in the fluorescence was determined by a low fraction of the excited states. Recovery was associated with large decrease in the entropy of activation that indicated the involvement of large structural rearrangements. Macroaggregated LHCII exhibited larger ΔF than small aggregates, which is consistent with the proposed role of aggregated LHCII in thylakoid membranes in nonphotochemical quenching.

Original languageEnglish
Pages (from-to)751-759
Number of pages9
JournalPhotochemistry and Photobiology
Volume70
Issue number5
Publication statusPublished - Nov 1999

Fingerprint

Photosystem II Protein Complex
chlorophylls
pigments
Pigments
Quenching
Fluorescence
quenching
proteins
Light
fluorescence
Kinetics
kinetics
quantitative analysis
recovery
Thylakoids
Recovery
Photoperiod
Entropy
chlorophyll a
chlorophyll b

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Kinetic analysis of the light-induced fluorescence quenching in light-harvesting chlorophyll a/b pigment-protein complex of photosystem II. / Barzda, Virginijus; Jennings, Robert C.; Zucchelli, Giuseppe; Garab, Gyözö.

In: Photochemistry and Photobiology, Vol. 70, No. 5, 11.1999, p. 751-759.

Research output: Contribution to journalArticle

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