Killer cells

a functional comparison between natural, immune T cell and antibody dependent in vitro systems

R. Kiessling, G. Petrányi, K. Karre, M. Jondal, D. Tracey, H. Wigzell

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Previous reports have shown that spleen cells from nonimmune adult mice of certain strains do regularly kill Moloney leukemia virus induced lymphomas in short term 51Cr release assays. This naturally occurring killer (NK) cell had low adherent properties and had the morphological appearance of a lymphocyte. Still it lacked surface characteristics of mature T or B lymphocytes. In the present report a functional study was carried out, comparing in parallel the NK system, the T cell killing across an H 2 barrier (anti P815), and the antibody dependent cell mediated chicken red blood cell (CRBC) system. In contrast to the effector cells in the CRBC system, the NK cells were insensitive to erythrocyte antibody complement (EAC) rosette depletion and would pass through nylon wool columns. NK activity was not inhibited by the presence of heat aggregated human or mouse gamma globulin, in contrast to the strong inhibition noted in the CRBC system. Sensitivity to trypsin pretreatment was noted in the NK system as well as in the immune P815 system, whereas the CRBC system was relatively trypsin resistant. Antitheta plus complement eliminated the anti P815 activity, but did not touch the NK activity. The present results thus further distinguish the NK cell from cytotoxic T lymphocytes or from antibody dependent killer cells.

Original languageEnglish
Pages (from-to)772-780
Number of pages9
JournalJournal of Experimental Medicine
Volume143
Issue number4
DOIs
Publication statusPublished - 1976

Fingerprint

T-Lymphocytes
Antibodies
Erythrocytes
Chickens
Trypsin
Moloney murine leukemia virus
Wool
gamma-Globulins
Nylons
Touch
Cytotoxic T-Lymphocytes
In Vitro Techniques
Anti-Idiotypic Antibodies
Immune System
Lymphoma
B-Lymphocytes
Spleen
Hot Temperature
Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Killer cells : a functional comparison between natural, immune T cell and antibody dependent in vitro systems. / Kiessling, R.; Petrányi, G.; Karre, K.; Jondal, M.; Tracey, D.; Wigzell, H.

In: Journal of Experimental Medicine, Vol. 143, No. 4, 1976, p. 772-780.

Research output: Contribution to journalArticle

Kiessling, R. ; Petrányi, G. ; Karre, K. ; Jondal, M. ; Tracey, D. ; Wigzell, H. / Killer cells : a functional comparison between natural, immune T cell and antibody dependent in vitro systems. In: Journal of Experimental Medicine. 1976 ; Vol. 143, No. 4. pp. 772-780.
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