Keratinocyte transglutaminase expression varies in squamous cell carcinomas

Madeleine Duvic, Deidra Chema Nelson, Mary Annarella, Mimi Cho, Teresa Esgleyes-Ribot, E. Remenyik, Roseann Ulmer, Ronald P. Rapini, Peter G. Sacks, Gary L. Clayman, Peter J A Davies, Scott Thacher

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Type I transglutaminase (TGase I, keratinocyte or particulate transglutaminase) is a 92-kilodalton (kDa) protein expressed in abundance in cultured keratinocytes and in the hyperproliferative skin disorder psoriasis. To determine the expression of TGase I protein and mRNA, we studied tissue and established squamous carcinoma lines derived from different sources. Immunohistochemistry and Western blotting were used to detect TGase I protein with the B.C1 mouse monoclonal antibody. Only well-differentiated, skin-derived squamous carcinomas stained for TGase I. However, a precocious pattern of expression was seen overlying less-differentiated tumors. Compared to cultured human keratinocytes, squamous cell carcinoma (SCC) had many times less to 7.8 times more TGase I protein, greatest in the two most differentiated tumor lines 14-83 and ME-180. TGase I mRNA levels ranged from 0.010 to 0.00004 pg/μg total RNA by reverse transcriptase-polymerase chain reaction using an internal standard. Protein expression correlated with mRNA levels in most SCC lines. When a human TGase I promoter was isolated and used to study genomic DNA, SCC 1-83 was shown to have unique restriction enzyme fragments, including one indicative of methylation differences, also present within DNA from the KB line. These studies suggest that transcriptional control of TGase I gene expression in squamous carcinomas may be influenced both by cis elements in the promoter and by the degree of tumor squamous differentiation.

Original languageEnglish
Pages (from-to)462-469
Number of pages8
JournalJournal of Investigative Dermatology
Volume102
Issue number4
Publication statusPublished - Apr 1994

Fingerprint

Transglutaminases
Keratinocytes
Squamous Cell Carcinoma
Proteins
Messenger RNA
Tumors
Skin
Methylation
Polymerase chain reaction
RNA-Directed DNA Polymerase
DNA
Gene expression
Carcinogens
Reverse Transcriptase Polymerase Chain Reaction
Psoriasis
Monoclonal Antibodies
Epithelial Cells
RNA
Neoplasms
Tissue

Keywords

  • cornified envelope
  • epidermal differentiation
  • epidermis
  • skin cancer

ASJC Scopus subject areas

  • Dermatology

Cite this

Duvic, M., Nelson, D. C., Annarella, M., Cho, M., Esgleyes-Ribot, T., Remenyik, E., ... Thacher, S. (1994). Keratinocyte transglutaminase expression varies in squamous cell carcinomas. Journal of Investigative Dermatology, 102(4), 462-469.

Keratinocyte transglutaminase expression varies in squamous cell carcinomas. / Duvic, Madeleine; Nelson, Deidra Chema; Annarella, Mary; Cho, Mimi; Esgleyes-Ribot, Teresa; Remenyik, E.; Ulmer, Roseann; Rapini, Ronald P.; Sacks, Peter G.; Clayman, Gary L.; Davies, Peter J A; Thacher, Scott.

In: Journal of Investigative Dermatology, Vol. 102, No. 4, 04.1994, p. 462-469.

Research output: Contribution to journalArticle

Duvic, M, Nelson, DC, Annarella, M, Cho, M, Esgleyes-Ribot, T, Remenyik, E, Ulmer, R, Rapini, RP, Sacks, PG, Clayman, GL, Davies, PJA & Thacher, S 1994, 'Keratinocyte transglutaminase expression varies in squamous cell carcinomas', Journal of Investigative Dermatology, vol. 102, no. 4, pp. 462-469.
Duvic M, Nelson DC, Annarella M, Cho M, Esgleyes-Ribot T, Remenyik E et al. Keratinocyte transglutaminase expression varies in squamous cell carcinomas. Journal of Investigative Dermatology. 1994 Apr;102(4):462-469.
Duvic, Madeleine ; Nelson, Deidra Chema ; Annarella, Mary ; Cho, Mimi ; Esgleyes-Ribot, Teresa ; Remenyik, E. ; Ulmer, Roseann ; Rapini, Ronald P. ; Sacks, Peter G. ; Clayman, Gary L. ; Davies, Peter J A ; Thacher, Scott. / Keratinocyte transglutaminase expression varies in squamous cell carcinomas. In: Journal of Investigative Dermatology. 1994 ; Vol. 102, No. 4. pp. 462-469.
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