Karyotypic analysis of Triticum monococcum using standard repetitive DNA probes and simple sequence repeats

M. Megyeri, A. Farkas, M. Varga, G. Kovács, M. Molnár-Láng, I. Molnár

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Triticum monococcum represents an important source of useful genes and alleles that it would be desirable to use in wheat breeding programmes. The well-defined landmarks on the Am chromosomes could accelerate the targeted introgression of T. monococcum chromatin into the wheat genome.Fluorescence in situ hybridization (FISH) using the repetitive DNA probes pSc119.2, Afa family and pTa71 showed that the pSc119.2 probe was not suitable for the identification of Am chromosomes. In contrast, the whole set of Am chromosomes (especially chromosomes 1, 4, 5 and 7) could be discriminated based on the hybridization pattern of pTa71 and Afa family. In situ hybridization with microsatellite motifs (GAA, CAG, AAC and AGG) proved that SSRs represent additional landmarks for the identification of Am chromosomes. The most promising SSR probes were the GAA and CAG motifs, which clearly discriminated the 6Am chromosome and, when used in combination with the Afa family and pTa71 probes, allowed the whole set of Am chromosomes to be reliably identified.In conclusion, fluorescence in situ hybridization using the repetitive DNA probes Afa family and pTa71, combined with SSR probes, makes it possible to identify the Am chromosomes of T. monococcum and to discriminate them from Au chromosomes in the polyploid wheat background.

Original languageEnglish
Pages (from-to)87-95
Number of pages9
JournalActa Agronomica Hungarica
Volume60
Issue number2
DOIs
Publication statusPublished - Jun 1 2012

Fingerprint

Triticum monococcum
DNA probes
microsatellite repeats
chromosomes
fluorescence in situ hybridization
wheat
polyploidy
introgression
in situ hybridization
chromatin
hybridization
alleles

Keywords

  • FISH
  • karyotypic analysis
  • microsatellite
  • Triticum monococcum

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Karyotypic analysis of Triticum monococcum using standard repetitive DNA probes and simple sequence repeats. / Megyeri, M.; Farkas, A.; Varga, M.; Kovács, G.; Molnár-Láng, M.; Molnár, I.

In: Acta Agronomica Hungarica, Vol. 60, No. 2, 01.06.2012, p. 87-95.

Research output: Contribution to journalArticle

@article{225c86258b5f4829b2835e206defc2de,
title = "Karyotypic analysis of Triticum monococcum using standard repetitive DNA probes and simple sequence repeats",
abstract = "Triticum monococcum represents an important source of useful genes and alleles that it would be desirable to use in wheat breeding programmes. The well-defined landmarks on the Am chromosomes could accelerate the targeted introgression of T. monococcum chromatin into the wheat genome.Fluorescence in situ hybridization (FISH) using the repetitive DNA probes pSc119.2, Afa family and pTa71 showed that the pSc119.2 probe was not suitable for the identification of Am chromosomes. In contrast, the whole set of Am chromosomes (especially chromosomes 1, 4, 5 and 7) could be discriminated based on the hybridization pattern of pTa71 and Afa family. In situ hybridization with microsatellite motifs (GAA, CAG, AAC and AGG) proved that SSRs represent additional landmarks for the identification of Am chromosomes. The most promising SSR probes were the GAA and CAG motifs, which clearly discriminated the 6Am chromosome and, when used in combination with the Afa family and pTa71 probes, allowed the whole set of Am chromosomes to be reliably identified.In conclusion, fluorescence in situ hybridization using the repetitive DNA probes Afa family and pTa71, combined with SSR probes, makes it possible to identify the Am chromosomes of T. monococcum and to discriminate them from Au chromosomes in the polyploid wheat background.",
keywords = "FISH, karyotypic analysis, microsatellite, Triticum monococcum",
author = "M. Megyeri and A. Farkas and M. Varga and G. Kov{\'a}cs and M. Moln{\'a}r-L{\'a}ng and I. Moln{\'a}r",
year = "2012",
month = "6",
day = "1",
doi = "10.1556/AAgr.60.2012.2.1",
language = "English",
volume = "60",
pages = "87--95",
journal = "Acta Agronomica Hungarica",
issn = "0238-0161",
publisher = "Akademiai Kiado",
number = "2",

}

TY - JOUR

T1 - Karyotypic analysis of Triticum monococcum using standard repetitive DNA probes and simple sequence repeats

AU - Megyeri, M.

AU - Farkas, A.

AU - Varga, M.

AU - Kovács, G.

AU - Molnár-Láng, M.

AU - Molnár, I.

PY - 2012/6/1

Y1 - 2012/6/1

N2 - Triticum monococcum represents an important source of useful genes and alleles that it would be desirable to use in wheat breeding programmes. The well-defined landmarks on the Am chromosomes could accelerate the targeted introgression of T. monococcum chromatin into the wheat genome.Fluorescence in situ hybridization (FISH) using the repetitive DNA probes pSc119.2, Afa family and pTa71 showed that the pSc119.2 probe was not suitable for the identification of Am chromosomes. In contrast, the whole set of Am chromosomes (especially chromosomes 1, 4, 5 and 7) could be discriminated based on the hybridization pattern of pTa71 and Afa family. In situ hybridization with microsatellite motifs (GAA, CAG, AAC and AGG) proved that SSRs represent additional landmarks for the identification of Am chromosomes. The most promising SSR probes were the GAA and CAG motifs, which clearly discriminated the 6Am chromosome and, when used in combination with the Afa family and pTa71 probes, allowed the whole set of Am chromosomes to be reliably identified.In conclusion, fluorescence in situ hybridization using the repetitive DNA probes Afa family and pTa71, combined with SSR probes, makes it possible to identify the Am chromosomes of T. monococcum and to discriminate them from Au chromosomes in the polyploid wheat background.

AB - Triticum monococcum represents an important source of useful genes and alleles that it would be desirable to use in wheat breeding programmes. The well-defined landmarks on the Am chromosomes could accelerate the targeted introgression of T. monococcum chromatin into the wheat genome.Fluorescence in situ hybridization (FISH) using the repetitive DNA probes pSc119.2, Afa family and pTa71 showed that the pSc119.2 probe was not suitable for the identification of Am chromosomes. In contrast, the whole set of Am chromosomes (especially chromosomes 1, 4, 5 and 7) could be discriminated based on the hybridization pattern of pTa71 and Afa family. In situ hybridization with microsatellite motifs (GAA, CAG, AAC and AGG) proved that SSRs represent additional landmarks for the identification of Am chromosomes. The most promising SSR probes were the GAA and CAG motifs, which clearly discriminated the 6Am chromosome and, when used in combination with the Afa family and pTa71 probes, allowed the whole set of Am chromosomes to be reliably identified.In conclusion, fluorescence in situ hybridization using the repetitive DNA probes Afa family and pTa71, combined with SSR probes, makes it possible to identify the Am chromosomes of T. monococcum and to discriminate them from Au chromosomes in the polyploid wheat background.

KW - FISH

KW - karyotypic analysis

KW - microsatellite

KW - Triticum monococcum

UR - http://www.scopus.com/inward/record.url?scp=84863587429&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863587429&partnerID=8YFLogxK

U2 - 10.1556/AAgr.60.2012.2.1

DO - 10.1556/AAgr.60.2012.2.1

M3 - Article

AN - SCOPUS:84863587429

VL - 60

SP - 87

EP - 95

JO - Acta Agronomica Hungarica

JF - Acta Agronomica Hungarica

SN - 0238-0161

IS - 2

ER -