Kainate-induced pial arteriolar dilation remains intact after cerebral ischemia in piglets

T. M. Louis, F. Bari, D. W. Busija

Research output: Contribution to journalArticle

Abstract

Activation of N-methyl-D-aspartate (NMDA) and kainate (KAI) receptors dilates cerebral arterioles. In a previous study, we found that cerebral vasodilation to NMDA is reduced after hypoxic/anoxic insults (AJP 270: H1225, 1996 & Stroke 27:1634, 1996). The purpose of this study was to examine the effects of ischemia on cerebral arteriolar dilation to KAI. Pial arteriolar diameter was determined in anesthetized piglets via intravital microscopy. Baseline arteriolar diameters were ∼ 100 μm. Cerebral ischemia was induced by raising intracranial pressure for 10 min. Topical application of KAI at concentrations of 5×10-5 and 10-4 mol/l dilated pial arterioles by 16±2% and 28±3% above baseline, respectively (p-5 mol/l), a NMDA antagonist, did not influence responses to KAI (13±3% and 25±3%; p>0.05, n=4). One hour after ischemia, pial arteriolar reactivity to KAI was not substantially modified (16±4% and 21±5%; p>0.05, n=6). Application of KAI after ischemia resulted in edema formation by 2-4 hours. We conclude that reduced NMDA- but intact KAI-vascular responsiveness would alter glutamate influences on the cerebral circulation after ischemic stress.

Original languageEnglish
JournalFASEB Journal
Volume11
Issue number3
Publication statusPublished - 1997

Fingerprint

Kainic Acid
aspartic acid
ischemia
Brain Ischemia
piglets
Dilatation
N-Methylaspartate
Arterioles
vasodilation
Cerebrovascular Circulation
topical application
Ischemia
stroke
blood vessels
glutamates
edema
Kainic Acid Receptors
antagonists
microscopy
Intracranial Pressure

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Kainate-induced pial arteriolar dilation remains intact after cerebral ischemia in piglets. / Louis, T. M.; Bari, F.; Busija, D. W.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

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