Japanese eel (Anguilla japonica Temminck & Schlegel, 1846) propagation using cryopreserved sperm samples

T. Müller, H. Matsubara, Y. Kubara, A. Horváth, J. F. Asturiano, B. Urbányi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The aim of this study was to test the artificial seminal plasma (ASP) as the extender as well as methanol as the cryoprotectant for cryopreservation, and to collect information on the fertilizing capacity of cryopreserved sperm samples. Eggs from Japanese eel, Anguilla japonica, females were control-fertilized with native sperm diluted with ASP and cryopreserved sperm (ASP as extender [in 1:100 ratio] and 10% methanol in v/v final concentration). No statistical differences (p < .05) were among the measured parameters (hatching, survival after 10 days post-hatch, malformation rates) between the two groups, thus this cryopreservation method can be used effectively for artificial propagation of the Japanese eel, Anguilla japonica.

Original languageEnglish
JournalJournal of Applied Ichthyology
DOIs
Publication statusAccepted/In press - 2017

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Anguilla japonica
eel
sperm
cryopreservation
seminal plasma
spermatozoa
plasma
methanol
sampling
hatching
cryoprotectants
egg
testing

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Japanese eel (Anguilla japonica Temminck & Schlegel, 1846) propagation using cryopreserved sperm samples. / Müller, T.; Matsubara, H.; Kubara, Y.; Horváth, A.; Asturiano, J. F.; Urbányi, B.

In: Journal of Applied Ichthyology, 2017.

Research output: Contribution to journalArticle

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AU - Kubara, Y.

AU - Horváth, A.

AU - Asturiano, J. F.

AU - Urbányi, B.

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