The degree of polarization of fluorescence in aqueous glycerol solutions of trypaflavine quenched by rhodamine B has been studied in the concentration range 6·10-5 to 2·10-3 mole/l of quencher, viscosities between 0.01 to 20 Poise and temperatures from 0°C to 50°C. Perrin-Levshin's relation of rotational depolarization has been found valid. The molar volume of trypaflavine derived from this relation was found to increase with increasing quencher concentration and viscosity. This has been explained by a rearrangement of the solvent layer around the excited molecule. The dependence of the molar volume on the mean lifetime of excitation permits conclusions on the kinetics of formation of the solvation shell.
ASJC Scopus subject areas
- Nuclear and High Energy Physics