Isoproterenol inhibits IL-10, TNF-α, and nitric oxide production in RAW 264.7 Macrophages

G. Haskó, Zoltán H. Németh, Csaba Szabó, G. Zsilla, Andrew L. Salzman, E. Vízi

Research output: Contribution to journalArticle

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Abstract

In a previous study we have demonstrated in conscious endotoxemic mice that isoproterenol, a nonselective agonist of β-adrenergic receptors, decreased the production of proinflammatory mediators tumor necrosis factor- α (TNF-α) and nitric oxide (NO), and enhanced the formation of the anti- inflammatory cytokine interleukin-10 (IL-10). In the present study we investigated the effect of isoproterenol on the bacterial lipopolysaccharide (endotoxin, LPS; 10 μg/ml)-induced inflammatory response in RAW 264.7 macrophages in vitro. Pretreatment of cells with isoproterenol (10-300 μM) resulted in an inhibition of TNF-α, NO (reflected as its stable breakdown product nitrite), as well as IL-10 production that was paralleled with a restoration of the LPS-induced suppression of mitochondrial respiration. In addition, isoproterenol elevated cAMP accumulation in these cells. Finally, isoproterenol (300 μM) did not influence the nuclear translocation of nuclear factor κB. These data demonstrate that isoproterenol potently downregulates the LPS-induced inflammatory response and further support the notion that stimulation of β-adrenoreceptors can be an effective strategy in the treatment of inflammatory diseases.

Original languageEnglish
Pages (from-to)183-187
Number of pages5
JournalBrain Research Bulletin
Volume45
Issue number2
DOIs
Publication statusPublished - 1998

Fingerprint

Isoproterenol
Interleukin-10
Nitric Oxide
Tumor Necrosis Factor-alpha
Macrophages
Adrenergic Agonists
Nitrites
Endotoxins
Lipopolysaccharides
Respiration
Anti-Inflammatory Agents
Down-Regulation
Cytokines

Keywords

  • β-Adrenoceptors
  • Catecholamines
  • Cyclic adenosine monophosphate
  • Cytokines
  • Inflammation
  • Lipopolysaccharide
  • Nitric Oxide
  • Septic shock

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Isoproterenol inhibits IL-10, TNF-α, and nitric oxide production in RAW 264.7 Macrophages. / Haskó, G.; Németh, Zoltán H.; Szabó, Csaba; Zsilla, G.; Salzman, Andrew L.; Vízi, E.

In: Brain Research Bulletin, Vol. 45, No. 2, 1998, p. 183-187.

Research output: Contribution to journalArticle

Haskó, G. ; Németh, Zoltán H. ; Szabó, Csaba ; Zsilla, G. ; Salzman, Andrew L. ; Vízi, E. / Isoproterenol inhibits IL-10, TNF-α, and nitric oxide production in RAW 264.7 Macrophages. In: Brain Research Bulletin. 1998 ; Vol. 45, No. 2. pp. 183-187.
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