Isolation of uracil auxotrophic mutants of Trichoderma harzianum and their transformation with heterologous vectors

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Nine uracil auxotrophic mutants were isolated from a mycoparasitic strain of Trichoderma harzianum. One of these could be transformed with the pKIM7 cosmid, which contained the pyr-4 gene of Neurospora crassa. Another uracil-requiring mutant was transformed with heterologous vectors containing the orotate pyrophosphoribosyl transferase genes of Podospora anserina and Trichoderma reesei. The transformation frequencies varied between 150 and 1000 μg-1 plasmid DNA. Hybridization analysis of the transformants revealed distinct locations of integrated vectors in the genomes, but in some transformants freely replicating plasmids could also be detected.

Original languageEnglish
Pages (from-to)59-62
Number of pages4
JournalFEMS microbiology letters
Volume130
Issue number1
DOIs
Publication statusPublished - Jul 15 1995

Keywords

  • Genetic transformation
  • Heterologous vectors
  • Trichoderma harzianum
  • Uracil auxotrophic mutants

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Isolation of uracil auxotrophic mutants of Trichoderma harzianum and their transformation with heterologous vectors'. Together they form a unique fingerprint.

  • Cite this