A procedure is described for the purification of the ribosomal RNA genes of Salmonella typhimurium LT‐2. Its steps are: ultrasonic fragmentation of the denatured DNA, separation of the complementary strands, hybridization of the rRNA transcribing strand with rRNA, and separation of the hybrid from bulk DNA by means of repeated chromatography on deoxycholate‐treated benzoylated‐DEAE‐cellulose. The purification was 190‐fold with approximately 6% yield. The purity of the final product was 64%.
|Number of pages||5|
|Journal||European Journal of Biochemistry|
|Publication status||Published - Jun 1971|
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