Isolation of the Ribosomal RNA Genes of Salmonella typhimurium

Andor Udvardy, Pál Venetianer

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Abstract

A procedure is described for the purification of the ribosomal RNA genes of Salmonella typhimurium LT‐2. Its steps are: ultrasonic fragmentation of the denatured DNA, separation of the complementary strands, hybridization of the rRNA transcribing strand with rRNA, and separation of the hybrid from bulk DNA by means of repeated chromatography on deoxycholate‐treated benzoylated‐DEAE‐cellulose. The purification was 190‐fold with approximately 6% yield. The purity of the final product was 64%.

Original languageEnglish
Pages (from-to)513-517
Number of pages5
JournalEuropean Journal of Biochemistry
Volume20
Issue number4
DOIs
Publication statusPublished - Jun 1971

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ASJC Scopus subject areas

  • Biochemistry

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