Isolation of the gene encoding the bovine neonatal Fc receptor

Márton Doleschall, Yaofeng Zhao, B. Mayer, Lennart Hammarström, I. Kacskovics

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The role of an IgG-Fc receptor (FcRn) that resembles a class I MHC Ag in transporting IgGs through epithelial cells was recently shown in selected species. Here we report our preliminary characterization of a clone encoding the alpha chain of the bovine FcRn from a BAC library. The recombinant BAC DNA was digested, analyzed by Southern blot hybridization and a bovine FcRn positive 9 kb long fragment was subcloned and partially sequenced. The exon/intron organization of the bovine FcRn alpha chain gene was deduced by comparison with its cDNA sequence. The sequence revealed a similar organization to the human and mouse FcRn alpha chain genes. The bovine FcRn alpha chain gene has acquired several repetitive sequences in its 5′-flanking region, including multiple SINE and LINE elements. Potential binding sites for transcription factors within the 5′-flanking sequence were identified using TESS and TFSEARCH programs. The 5′-flanking region of the bFcRn alpha chain gene was analyzed for its ability to directly express the luciferase reporter gene in bovine mammary gland epithelial cells. Transient transfection of a luciferase construct revealed that there was promoter activity in the region -1787 to +92 of the 5′-flanking sequence.

Original languageEnglish
Pages (from-to)145-150
Number of pages6
JournalVeterinary Immunology and Immunopathology
Volume108
Issue number1-2 SPEC. ISS.
DOIs
Publication statusPublished - Oct 18 2005

Fingerprint

5' Flanking Region
receptors
cattle
Genes
luciferase
Luciferases
genes
epithelial cells
Short Interspersed Nucleotide Elements
Epithelial Cells
IgG Receptors
recombinant DNA
repetitive sequences
Recombinant DNA
Fc Receptors
Nucleic Acid Repetitive Sequences
transfection
Human Mammary Glands
Southern Blotting
Reporter Genes

Keywords

  • Bovine Fc receptor
  • Gene regulation
  • Immunoglobulin
  • Maternal antibodies
  • Mucosal immunity

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Cite this

Isolation of the gene encoding the bovine neonatal Fc receptor. / Doleschall, Márton; Zhao, Yaofeng; Mayer, B.; Hammarström, Lennart; Kacskovics, I.

In: Veterinary Immunology and Immunopathology, Vol. 108, No. 1-2 SPEC. ISS., 18.10.2005, p. 145-150.

Research output: Contribution to journalArticle

Doleschall, Márton ; Zhao, Yaofeng ; Mayer, B. ; Hammarström, Lennart ; Kacskovics, I. / Isolation of the gene encoding the bovine neonatal Fc receptor. In: Veterinary Immunology and Immunopathology. 2005 ; Vol. 108, No. 1-2 SPEC. ISS. pp. 145-150.
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