Isolation of pepsin-resistant laminin fragments from human placenta: Effect on epithelial cells cultured from the kidneys of patients with autosomal dominant polycystic kidney disease (ADPKD)

Martin J. Slade, I. Pócsi, Ruth B. Kirby, J. Kay Jones, Susan E. Ganz, Sarah A. Taylor, Robert G. Price

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Laminin isolated from human placenta was subjected to prolonged pepsin digestion. Seven peptide fragments (designated N1 to N7) were separated by ion-exchange chromatography and gel filtration and characterised by SDS-polyacrylamide gel electrophoresis and immunoblotting. The molecular size of the laminin fragments varied from approx. 900,000 (N1) to 28,000 (N7). Epithelial cells obtained from normal kidneys and patients with autosomal dominant polycystic kidney disease (ADPKD) were cultured. The incorporation of [3H]thymidine was measured over 96 h to determine the effect of the addition of the different fragments and whole laminin from EHS tumour to the cells. The rate of growth of both normal and polycystic cells was increased in the presence of the laminin fragments but this effect was more pronounced in the ADPKD cells.

Original languageEnglish
Pages (from-to)25-31
Number of pages7
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1310
Issue number1
DOIs
Publication statusPublished - Jan 10 1996

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Autosomal Dominant Polycystic Kidney
Laminin
Placenta
Epithelial Cells
Kidney
Experimental Sarcomas
Peptide Fragments
Pepsin A
Ion Exchange Chromatography
Immunoblotting
Thymidine
Gel Chromatography
Polyacrylamide Gel Electrophoresis
Digestion
laminin P1
Growth

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biophysics

Cite this

Isolation of pepsin-resistant laminin fragments from human placenta : Effect on epithelial cells cultured from the kidneys of patients with autosomal dominant polycystic kidney disease (ADPKD). / Slade, Martin J.; Pócsi, I.; Kirby, Ruth B.; Jones, J. Kay; Ganz, Susan E.; Taylor, Sarah A.; Price, Robert G.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1310, No. 1, 10.01.1996, p. 25-31.

Research output: Contribution to journalArticle

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