Isolation of Mycoplasma capricolum-like strains from chickens

Dušan Benčina, Janet M. Bradbury, L. Stipkovits, Zsuzsana Varga, Andrej Razpet, Andrej Bidovec, Peter Dovč

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Members of the genus Mycoplasma infect a wide range of hosts, but individual Mycoplasma species tend to exhibit a considerable degree of host specificity. We characterized Mycoplasma strain 700, isolated from a kidney of a layer hen in Spain and Mycoplasma strains ULB-A and ULB-B, isolated from the air sac and from the bile of stunted broiler chickens in Slovenia. The serologic examination showed that these three strains are antigenically unrelated to all of the recognized Mycoplasma species of avian origin, but closely related to the ruminant mycoplasma Mycoplasma capricolum subspecies capricolum (M. capricolum). The comparison of their 16S rRNA gene sequences with the sequence of M. capricolum (California kid) revealed 99.66% sequence identity for the strain 700 and 99.59% identity for strains ULB-A and ULB-B. Moreover, the predicted DnaK sequences of the M. capricolum-like strains, isolated from chickens, were identical to DnaK sequences of M. capricolum. Comparison of their dnaK gene sequences with M. capricolum showed 99.64% sequence identity for strain 700 and 99.27% identity for strains ULB-A and ULB-B. In the flock from which M. capricolum-like strains ULB-A and ULB-B were isolated, the majority of chickens (83% of the chickens examined) raised antibodies reacting with M. capricolum antigens. Notably, the infection of chickens with M. capricolum-like strains represents an unusual exception to the range of Mycoplasma species host specificity.

Original languageEnglish
Pages (from-to)23-31
Number of pages9
JournalVeterinary Microbiology
Volume112
Issue number1
DOIs
Publication statusPublished - Jan 10 2006

Fingerprint

Mycoplasma capricolum
Mycoplasma
Chickens
chickens
Host Specificity
host specificity
Air Sacs
Slovenia
Ruminants
nucleotide sequences
air sacs
rRNA Genes
Bile
Spain
kids (goats)
bile
host range
laying hens
ruminants

Keywords

  • 16S rRNA and dnaK sequence analysis
  • Antigenic comparison
  • Chicken
  • Mycoplasma capricolum

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

Benčina, D., Bradbury, J. M., Stipkovits, L., Varga, Z., Razpet, A., Bidovec, A., & Dovč, P. (2006). Isolation of Mycoplasma capricolum-like strains from chickens. Veterinary Microbiology, 112(1), 23-31. https://doi.org/10.1016/j.vetmic.2005.10.001

Isolation of Mycoplasma capricolum-like strains from chickens. / Benčina, Dušan; Bradbury, Janet M.; Stipkovits, L.; Varga, Zsuzsana; Razpet, Andrej; Bidovec, Andrej; Dovč, Peter.

In: Veterinary Microbiology, Vol. 112, No. 1, 10.01.2006, p. 23-31.

Research output: Contribution to journalArticle

Benčina, D, Bradbury, JM, Stipkovits, L, Varga, Z, Razpet, A, Bidovec, A & Dovč, P 2006, 'Isolation of Mycoplasma capricolum-like strains from chickens', Veterinary Microbiology, vol. 112, no. 1, pp. 23-31. https://doi.org/10.1016/j.vetmic.2005.10.001
Benčina, Dušan ; Bradbury, Janet M. ; Stipkovits, L. ; Varga, Zsuzsana ; Razpet, Andrej ; Bidovec, Andrej ; Dovč, Peter. / Isolation of Mycoplasma capricolum-like strains from chickens. In: Veterinary Microbiology. 2006 ; Vol. 112, No. 1. pp. 23-31.
@article{d50e4170ec324aa9a4dd3600559d06a7,
title = "Isolation of Mycoplasma capricolum-like strains from chickens",
abstract = "Members of the genus Mycoplasma infect a wide range of hosts, but individual Mycoplasma species tend to exhibit a considerable degree of host specificity. We characterized Mycoplasma strain 700, isolated from a kidney of a layer hen in Spain and Mycoplasma strains ULB-A and ULB-B, isolated from the air sac and from the bile of stunted broiler chickens in Slovenia. The serologic examination showed that these three strains are antigenically unrelated to all of the recognized Mycoplasma species of avian origin, but closely related to the ruminant mycoplasma Mycoplasma capricolum subspecies capricolum (M. capricolum). The comparison of their 16S rRNA gene sequences with the sequence of M. capricolum (California kid) revealed 99.66{\%} sequence identity for the strain 700 and 99.59{\%} identity for strains ULB-A and ULB-B. Moreover, the predicted DnaK sequences of the M. capricolum-like strains, isolated from chickens, were identical to DnaK sequences of M. capricolum. Comparison of their dnaK gene sequences with M. capricolum showed 99.64{\%} sequence identity for strain 700 and 99.27{\%} identity for strains ULB-A and ULB-B. In the flock from which M. capricolum-like strains ULB-A and ULB-B were isolated, the majority of chickens (83{\%} of the chickens examined) raised antibodies reacting with M. capricolum antigens. Notably, the infection of chickens with M. capricolum-like strains represents an unusual exception to the range of Mycoplasma species host specificity.",
keywords = "16S rRNA and dnaK sequence analysis, Antigenic comparison, Chicken, Mycoplasma capricolum",
author = "Dušan Benčina and Bradbury, {Janet M.} and L. Stipkovits and Zsuzsana Varga and Andrej Razpet and Andrej Bidovec and Peter Dovč",
year = "2006",
month = "1",
day = "10",
doi = "10.1016/j.vetmic.2005.10.001",
language = "English",
volume = "112",
pages = "23--31",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Isolation of Mycoplasma capricolum-like strains from chickens

AU - Benčina, Dušan

AU - Bradbury, Janet M.

AU - Stipkovits, L.

AU - Varga, Zsuzsana

AU - Razpet, Andrej

AU - Bidovec, Andrej

AU - Dovč, Peter

PY - 2006/1/10

Y1 - 2006/1/10

N2 - Members of the genus Mycoplasma infect a wide range of hosts, but individual Mycoplasma species tend to exhibit a considerable degree of host specificity. We characterized Mycoplasma strain 700, isolated from a kidney of a layer hen in Spain and Mycoplasma strains ULB-A and ULB-B, isolated from the air sac and from the bile of stunted broiler chickens in Slovenia. The serologic examination showed that these three strains are antigenically unrelated to all of the recognized Mycoplasma species of avian origin, but closely related to the ruminant mycoplasma Mycoplasma capricolum subspecies capricolum (M. capricolum). The comparison of their 16S rRNA gene sequences with the sequence of M. capricolum (California kid) revealed 99.66% sequence identity for the strain 700 and 99.59% identity for strains ULB-A and ULB-B. Moreover, the predicted DnaK sequences of the M. capricolum-like strains, isolated from chickens, were identical to DnaK sequences of M. capricolum. Comparison of their dnaK gene sequences with M. capricolum showed 99.64% sequence identity for strain 700 and 99.27% identity for strains ULB-A and ULB-B. In the flock from which M. capricolum-like strains ULB-A and ULB-B were isolated, the majority of chickens (83% of the chickens examined) raised antibodies reacting with M. capricolum antigens. Notably, the infection of chickens with M. capricolum-like strains represents an unusual exception to the range of Mycoplasma species host specificity.

AB - Members of the genus Mycoplasma infect a wide range of hosts, but individual Mycoplasma species tend to exhibit a considerable degree of host specificity. We characterized Mycoplasma strain 700, isolated from a kidney of a layer hen in Spain and Mycoplasma strains ULB-A and ULB-B, isolated from the air sac and from the bile of stunted broiler chickens in Slovenia. The serologic examination showed that these three strains are antigenically unrelated to all of the recognized Mycoplasma species of avian origin, but closely related to the ruminant mycoplasma Mycoplasma capricolum subspecies capricolum (M. capricolum). The comparison of their 16S rRNA gene sequences with the sequence of M. capricolum (California kid) revealed 99.66% sequence identity for the strain 700 and 99.59% identity for strains ULB-A and ULB-B. Moreover, the predicted DnaK sequences of the M. capricolum-like strains, isolated from chickens, were identical to DnaK sequences of M. capricolum. Comparison of their dnaK gene sequences with M. capricolum showed 99.64% sequence identity for strain 700 and 99.27% identity for strains ULB-A and ULB-B. In the flock from which M. capricolum-like strains ULB-A and ULB-B were isolated, the majority of chickens (83% of the chickens examined) raised antibodies reacting with M. capricolum antigens. Notably, the infection of chickens with M. capricolum-like strains represents an unusual exception to the range of Mycoplasma species host specificity.

KW - 16S rRNA and dnaK sequence analysis

KW - Antigenic comparison

KW - Chicken

KW - Mycoplasma capricolum

UR - http://www.scopus.com/inward/record.url?scp=29144514799&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29144514799&partnerID=8YFLogxK

U2 - 10.1016/j.vetmic.2005.10.001

DO - 10.1016/j.vetmic.2005.10.001

M3 - Article

VL - 112

SP - 23

EP - 31

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 1

ER -