Isolation of chlorogenic acid from soil borne fungi Screlotium rolfsii, their reversal of multidrug resistance and anti-proliferative in mouse lymphoma cells

Bashir Ahmad, Muhammad Rizwan, Abdur Rauf, Muslim Raza, Shumaila Bashir, J. Molnár, Ákos Csonka, Diána Szabó, Mohammad S. Mubarak, Mah Noor, Bina S. Siddiqui

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Fungi performing a wide range of function in soil by secreting low molecular weight compound known as secondary metabolites. S. rolfsii is a soil borne phytopathogenic fungi was used for the production of bioactive compounds. Objective: The present study belongs to evaluate the anticancer potentials of a secondary metabolites isolated from S. rolfsii, their multidrug resistance (MDR), and molecular docking study. Method: (1S, 3R, 4R, 5R, E)-3-(3-(3, 4-Dihydroxyphenyl)acryloyloxy)-1, 4, 5 trihydroxycyclohexanecarboxylic acid (1), or best known as chlorogenic acid, was isolated from the ethyl acetate fraction of crude secondary metabolites produced by the soil borne Fungus Screlotium rolfsii. Structure of chlorogenic acid (1) was confirmed by means of FT-IR, 1H NMR, 13C NMR, and mass spectrometry as well as by melting point. Results: Effect of compound 1 on the reversion of multidrug resistant (MDR) mediated by Pglycoprotein (P-gp) against cancer cells was evaluated with a rhodamine-123 exclusion screening test on human mdr1 gene transfected mouse gene transfected L5178 and L5178Y mouse T-cell lymphoma. Compound 1 was also evaluated for Anti-proliferative effect on the L5178 mouse Tcell lymphoma cell line. Conclusion: Results from the present investigation revealed that compound 1 exhibits excellent MDR reversing effect in a dose-dependent manner against mouse T-lymphoma cell line. Compound 1 also showed anti-proliferative effect on L5178Y mouse T-lymphoma cell line.

Original languageEnglish
Pages (from-to)721-726
Number of pages6
JournalMedicinal Chemistry
Volume13
Issue number8
DOIs
Publication statusPublished - Dec 1 2017

Fingerprint

Chlorogenic Acid
Multiple Drug Resistance
Lymphoma
Fungi
Soil
Cell Line
Rhodamine 123
T-Cell Lymphoma
Freezing
Genes
Mass Spectrometry
Molecular Weight
Acids
Neoplasms

Keywords

  • Chlorogenic acid
  • Fungi
  • Glycoprotein
  • Multidrug Resistance (MDR)
  • Screlotium rolfsii
  • Secondary metabolites

ASJC Scopus subject areas

  • Drug Discovery

Cite this

Isolation of chlorogenic acid from soil borne fungi Screlotium rolfsii, their reversal of multidrug resistance and anti-proliferative in mouse lymphoma cells. / Ahmad, Bashir; Rizwan, Muhammad; Rauf, Abdur; Raza, Muslim; Bashir, Shumaila; Molnár, J.; Csonka, Ákos; Szabó, Diána; Mubarak, Mohammad S.; Noor, Mah; Siddiqui, Bina S.

In: Medicinal Chemistry, Vol. 13, No. 8, 01.12.2017, p. 721-726.

Research output: Contribution to journalArticle

Ahmad, Bashir ; Rizwan, Muhammad ; Rauf, Abdur ; Raza, Muslim ; Bashir, Shumaila ; Molnár, J. ; Csonka, Ákos ; Szabó, Diána ; Mubarak, Mohammad S. ; Noor, Mah ; Siddiqui, Bina S. / Isolation of chlorogenic acid from soil borne fungi Screlotium rolfsii, their reversal of multidrug resistance and anti-proliferative in mouse lymphoma cells. In: Medicinal Chemistry. 2017 ; Vol. 13, No. 8. pp. 721-726.
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abstract = "Background: Fungi performing a wide range of function in soil by secreting low molecular weight compound known as secondary metabolites. S. rolfsii is a soil borne phytopathogenic fungi was used for the production of bioactive compounds. Objective: The present study belongs to evaluate the anticancer potentials of a secondary metabolites isolated from S. rolfsii, their multidrug resistance (MDR), and molecular docking study. Method: (1S, 3R, 4R, 5R, E)-3-(3-(3, 4-Dihydroxyphenyl)acryloyloxy)-1, 4, 5 trihydroxycyclohexanecarboxylic acid (1), or best known as chlorogenic acid, was isolated from the ethyl acetate fraction of crude secondary metabolites produced by the soil borne Fungus Screlotium rolfsii. Structure of chlorogenic acid (1) was confirmed by means of FT-IR, 1H NMR, 13C NMR, and mass spectrometry as well as by melting point. Results: Effect of compound 1 on the reversion of multidrug resistant (MDR) mediated by Pglycoprotein (P-gp) against cancer cells was evaluated with a rhodamine-123 exclusion screening test on human mdr1 gene transfected mouse gene transfected L5178 and L5178Y mouse T-cell lymphoma. Compound 1 was also evaluated for Anti-proliferative effect on the L5178 mouse Tcell lymphoma cell line. Conclusion: Results from the present investigation revealed that compound 1 exhibits excellent MDR reversing effect in a dose-dependent manner against mouse T-lymphoma cell line. Compound 1 also showed anti-proliferative effect on L5178Y mouse T-lymphoma cell line.",
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AU - Rizwan, Muhammad

AU - Rauf, Abdur

AU - Raza, Muslim

AU - Bashir, Shumaila

AU - Molnár, J.

AU - Csonka, Ákos

AU - Szabó, Diána

AU - Mubarak, Mohammad S.

AU - Noor, Mah

AU - Siddiqui, Bina S.

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AB - Background: Fungi performing a wide range of function in soil by secreting low molecular weight compound known as secondary metabolites. S. rolfsii is a soil borne phytopathogenic fungi was used for the production of bioactive compounds. Objective: The present study belongs to evaluate the anticancer potentials of a secondary metabolites isolated from S. rolfsii, their multidrug resistance (MDR), and molecular docking study. Method: (1S, 3R, 4R, 5R, E)-3-(3-(3, 4-Dihydroxyphenyl)acryloyloxy)-1, 4, 5 trihydroxycyclohexanecarboxylic acid (1), or best known as chlorogenic acid, was isolated from the ethyl acetate fraction of crude secondary metabolites produced by the soil borne Fungus Screlotium rolfsii. Structure of chlorogenic acid (1) was confirmed by means of FT-IR, 1H NMR, 13C NMR, and mass spectrometry as well as by melting point. Results: Effect of compound 1 on the reversion of multidrug resistant (MDR) mediated by Pglycoprotein (P-gp) against cancer cells was evaluated with a rhodamine-123 exclusion screening test on human mdr1 gene transfected mouse gene transfected L5178 and L5178Y mouse T-cell lymphoma. Compound 1 was also evaluated for Anti-proliferative effect on the L5178 mouse Tcell lymphoma cell line. Conclusion: Results from the present investigation revealed that compound 1 exhibits excellent MDR reversing effect in a dose-dependent manner against mouse T-lymphoma cell line. Compound 1 also showed anti-proliferative effect on L5178Y mouse T-lymphoma cell line.

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KW - Multidrug Resistance (MDR)

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