Isolation of cDNA clones for genes that are differentially expressed during thermoinhibition in achenes of Tagetes minuta L.

Paul Hills, E. Balázs, Johannes Van Staden

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Differential display was used to isolate genes which are expressed specifically in thermoinhibited achenes of Tagetes minuta L. Of the 62 thermoinhibition-associated cDNAs identified, thirteen were cloned. Three clones for each cDNA were selected and reverse northern analysis used to confirm that all 39 clones were specific to the mRNA pool of thermoinhibited achenes only. High quality sequence data were obtained for 27 of these. Alignment of the various sequences revealed that a total of 14 different sequences had been cloned, indicating that several of the bands isolated from the differential display gels contained multiple sequences. Electronic homology searches tentatively identified three of the sequences, whilst the remainder did not show any significant homology to known sequences. Of the sequences identified, one may encode a plant transcription factor-like or nuclear RNA-binding protein, another an RNase-L inhibitor-like protein and the third a miraculin homologue. The potential roles of these genes in the imposition and maintenance of the thermoinhibited state are discussed. These results are consistent with the hypothesis that thermoinhibition in T. minuta achenes is under positive genetic control, in a manner analogous to embryo dormancy.

Original languageEnglish
Pages (from-to)83-94
Number of pages12
JournalPlant Growth Regulation
Volume45
Issue number1
DOIs
Publication statusPublished - Jan 2005

Fingerprint

Tagetes
Tagetes minuta
Complementary DNA
Clone Cells
clones
Nuclear RNA
fruits
RNA-Binding Proteins
Sequence Alignment
Nuclear Proteins
Genes
RNA-binding proteins
Transcription Factors
genes
Embryonic Structures
Gels
nuclear proteins
Maintenance
sequence alignment
embryo (plant)

Keywords

  • Differential display
  • Seed germination
  • Tagetes minuta L.
  • Thermoinhibition

ASJC Scopus subject areas

  • Plant Science

Cite this

Isolation of cDNA clones for genes that are differentially expressed during thermoinhibition in achenes of Tagetes minuta L. / Hills, Paul; Balázs, E.; Van Staden, Johannes.

In: Plant Growth Regulation, Vol. 45, No. 1, 01.2005, p. 83-94.

Research output: Contribution to journalArticle

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