Isolation of cDNA clones for genes that are differentially expressed during thermoinhibition in achenes of Tagetes minuta L.

Paul Hills, Ervin Balázs, Johannes Van Staden

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3 Citations (Scopus)

Abstract

Differential display was used to isolate genes which are expressed specifically in thermoinhibited achenes of Tagetes minuta L. Of the 62 thermoinhibition-associated cDNAs identified, thirteen were cloned. Three clones for each cDNA were selected and reverse northern analysis used to confirm that all 39 clones were specific to the mRNA pool of thermoinhibited achenes only. High quality sequence data were obtained for 27 of these. Alignment of the various sequences revealed that a total of 14 different sequences had been cloned, indicating that several of the bands isolated from the differential display gels contained multiple sequences. Electronic homology searches tentatively identified three of the sequences, whilst the remainder did not show any significant homology to known sequences. Of the sequences identified, one may encode a plant transcription factor-like or nuclear RNA-binding protein, another an RNase-L inhibitor-like protein and the third a miraculin homologue. The potential roles of these genes in the imposition and maintenance of the thermoinhibited state are discussed. These results are consistent with the hypothesis that thermoinhibition in T. minuta achenes is under positive genetic control, in a manner analogous to embryo dormancy.

Original languageEnglish
Pages (from-to)83-94
Number of pages12
JournalPlant Growth Regulation
Volume45
Issue number1
DOIs
Publication statusPublished - Jan 1 2005

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Keywords

  • Differential display
  • Seed germination
  • Tagetes minuta L.
  • Thermoinhibition

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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