A cDNA library was prepared from the immature kernels of an isogenic wheat line originating from Bánkúti 1201 in order to isolate LMW glutenin genes. After screening the library a chimeric gene was identified consisting of N-terminal gamma gliadin and C-terminal LMW glutenin fragments. All together ten recombinant genes with similar structure were isolated from the cDNA library and the genomic DNA using chimera-specific PCR primers. These genes exhibit great variability concerning their size, structure and cysteine residue content. Half of the clones contain frame-shift mutations modifying the size and amino acid sequence of the encoded proteins to a great extent. Functionally the chimeric proteins seem to be less diverse than from the structural point of view. Most of them seem to act as chain terminators in the dough. Further experimental efforts are needed to reveal the origin and the geographic and taxonomical distribution of this new gene family, as well as the physico-chemical characteristics of their protein products.
|Number of pages||8|
|Publication status||Published - Dec 1 2005|
ASJC Scopus subject areas
- Agronomy and Crop Science