Isolation of a new member of the ecdysteroid glycoside family

2-deoxy-20-hydroxyecdysone 22-O-β-D-glucopyranoside

M. Báthori, Zita Pongrácz, Gábor Tóth, A. Simon, L. Kandra, Z. Kele, R. Ohmacht

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A new ecdysteroid glycoside, 2-deoxy-20-hydroxyecdysone 22-O-β-D-glucopyranoside, is isolated from the herb Silene italica ssp. nemoralis (Waldst. and Kit.) Nyman. The compound is purified with multistep chromatography, such as classical column chromatography on alumina and droplet countercurrent distribution. Also, it is expanded using twice low-pressure reversed-phase liquid column chromatography. Chromatography in four steps results in the purified 2-deoxy-20-hydroxyecdysone 22-O-β-D-glucopyranoside. Two other ecdysteroids have also been separated, including the formerly identified integristerone A and 24(28)- dehvdromakisterone A.

Original languageEnglish
Pages (from-to)409-415
Number of pages7
JournalJournal of Chromatographic Science
Volume40
Issue number7
Publication statusPublished - Aug 2002

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Ecdysterone
Ecdysteroids
Column chromatography
Glycosides
Chromatography
Aluminum Oxide
Liquid chromatography
Silene
Countercurrent Distribution
Reverse-Phase Chromatography
Pressure
2-deoxy-20-hydroxyecdysone-22-O-glucopyranoside

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

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abstract = "A new ecdysteroid glycoside, 2-deoxy-20-hydroxyecdysone 22-O-β-D-glucopyranoside, is isolated from the herb Silene italica ssp. nemoralis (Waldst. and Kit.) Nyman. The compound is purified with multistep chromatography, such as classical column chromatography on alumina and droplet countercurrent distribution. Also, it is expanded using twice low-pressure reversed-phase liquid column chromatography. Chromatography in four steps results in the purified 2-deoxy-20-hydroxyecdysone 22-O-β-D-glucopyranoside. Two other ecdysteroids have also been separated, including the formerly identified integristerone A and 24(28)- dehvdromakisterone A.",
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T1 - Isolation of a new member of the ecdysteroid glycoside family

T2 - 2-deoxy-20-hydroxyecdysone 22-O-β-D-glucopyranoside

AU - Báthori, M.

AU - Pongrácz, Zita

AU - Tóth, Gábor

AU - Simon, A.

AU - Kandra, L.

AU - Kele, Z.

AU - Ohmacht, R.

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AB - A new ecdysteroid glycoside, 2-deoxy-20-hydroxyecdysone 22-O-β-D-glucopyranoside, is isolated from the herb Silene italica ssp. nemoralis (Waldst. and Kit.) Nyman. The compound is purified with multistep chromatography, such as classical column chromatography on alumina and droplet countercurrent distribution. Also, it is expanded using twice low-pressure reversed-phase liquid column chromatography. Chromatography in four steps results in the purified 2-deoxy-20-hydroxyecdysone 22-O-β-D-glucopyranoside. Two other ecdysteroids have also been separated, including the formerly identified integristerone A and 24(28)- dehvdromakisterone A.

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