Isolation, nucleotide sequence, and expression of a cDNA encoding pig citrate synthase

Claudia T. Evans, Daniel D. Owens, B. Sümegi, G. Kispál, P. Srere

Research output: Contribution to journalArticle

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Abstract

Citrate synthase is a key enzyme of the Krebs tricarboxylic acid cycle and catalyzes the stereospecific synthesis of citrate from acetyl coenzyme A and oxalacetate. The amino acid sequence and three-dimensional structure of pig citrate synthase dimers are known, and regions of the enzyme involved in substrate binding and catalysis have been identified. A cloned complementary DNA sequence encoding pig citrate synthase has been isolated from a pig kidney λgt11 cDNA library after screening with a synthetic oligonucleotide probe. The complete nucleotide sequence of the 1.5-kilobase cDNA was determined. The coding region consists of 1395 base pairs and confirms the amino acid sequence of purified pig citrate synthase. The derived amino acid sequence of pig citrate synthase predicts the presence of a 27 amino acid N-terminal leader peptide whose sequence is consistent with the sequences of other mitochondrial signal peptides. A conserved amino acid sequence in the mitochondrial leader peptides of pig citrate synthase and yeast mitochondrial citrate synthase was identified. To express the pig citrate synthase cDNA in Escherichia coli, we employed the inducible T7 RNA polymerase/promoter double plasmid expression vectors pGP1-2 and pT7-7 [Tabor, S., & Richardson, C. C. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 1074-1078]. The pig citrate synthase cDNA was modified to delete the N-terminal leader sequence; then by use of a synthetic oligonucleotide linker, the modified cDNA was cloned into pT7-7 immediately following the initiator Met. A glutamate-requiring (citrate synthase deficient), recA- E. coli mutant, DEK15, was transformed with pGPl-2 and then pT7-7PCS. pT7-7PCS complemented the E. coli gltA mutation. Following induction, the expressed pig citrate synthase protein was estimated to be about 9% of the total cell protein. Expression of the pig citrate synthase cDNA in E. coli yielded a functionally active enzyme that comigrated on sodium dodecyl sulfate-polyacrylamide gels with the purified pig heart protein.

Original languageEnglish
Pages (from-to)4680-4686
Number of pages7
JournalBiochemistry
Volume27
Issue number13
Publication statusPublished - 1988

Fingerprint

Citrate (si)-Synthase
Swine
Nucleotides
Complementary DNA
Escherichia coli
Amino Acid Sequence
Amino Acids
Protein Sorting Signals
Enzymes
Acetyl Coenzyme A
Proteins
Citric Acid Cycle
Oligonucleotide Probes
DNA sequences
Catalysis
Gene Library
Citric Acid
Oligonucleotides
Base Pairing
Sodium Dodecyl Sulfate

ASJC Scopus subject areas

  • Biochemistry

Cite this

Isolation, nucleotide sequence, and expression of a cDNA encoding pig citrate synthase. / Evans, Claudia T.; Owens, Daniel D.; Sümegi, B.; Kispál, G.; Srere, P.

In: Biochemistry, Vol. 27, No. 13, 1988, p. 4680-4686.

Research output: Contribution to journalArticle

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abstract = "Citrate synthase is a key enzyme of the Krebs tricarboxylic acid cycle and catalyzes the stereospecific synthesis of citrate from acetyl coenzyme A and oxalacetate. The amino acid sequence and three-dimensional structure of pig citrate synthase dimers are known, and regions of the enzyme involved in substrate binding and catalysis have been identified. A cloned complementary DNA sequence encoding pig citrate synthase has been isolated from a pig kidney λgt11 cDNA library after screening with a synthetic oligonucleotide probe. The complete nucleotide sequence of the 1.5-kilobase cDNA was determined. The coding region consists of 1395 base pairs and confirms the amino acid sequence of purified pig citrate synthase. The derived amino acid sequence of pig citrate synthase predicts the presence of a 27 amino acid N-terminal leader peptide whose sequence is consistent with the sequences of other mitochondrial signal peptides. A conserved amino acid sequence in the mitochondrial leader peptides of pig citrate synthase and yeast mitochondrial citrate synthase was identified. To express the pig citrate synthase cDNA in Escherichia coli, we employed the inducible T7 RNA polymerase/promoter double plasmid expression vectors pGP1-2 and pT7-7 [Tabor, S., & Richardson, C. C. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 1074-1078]. The pig citrate synthase cDNA was modified to delete the N-terminal leader sequence; then by use of a synthetic oligonucleotide linker, the modified cDNA was cloned into pT7-7 immediately following the initiator Met. A glutamate-requiring (citrate synthase deficient), recA- E. coli mutant, DEK15, was transformed with pGPl-2 and then pT7-7PCS. pT7-7PCS complemented the E. coli gltA mutation. Following induction, the expressed pig citrate synthase protein was estimated to be about 9{\%} of the total cell protein. Expression of the pig citrate synthase cDNA in E. coli yielded a functionally active enzyme that comigrated on sodium dodecyl sulfate-polyacrylamide gels with the purified pig heart protein.",
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