Isolation and Sequencing of a Genomic Clone for Mouse Brain Specific Small RNA

Kaijiro Anzai, Shunsuke Kobayashi, Narumi Kitamura, Yuri Kanai, Hiromichi Nakajima, Yoshioki Suehiro, Sataro Goto

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Abstract: We isolated a mouse genomic clone that hybridized with small RNA present in the cytoplasm of the brain. The RNA was about 150 nucleotides long. This RNA seemed to be specific to the brain, since it was not found in the liver or kidney. The clone DNA contained a sequence homologous to 82‐nucleotide “identifier” core sequence of cDNA clones of rat. The sequence contained a split promoter for RNA polymerase III and was flanked by a 12‐nucleotide direct repeat (ATAAATAATTTA).

Original languageEnglish
Pages (from-to)673-677
Number of pages5
JournalJournal of neurochemistry
Volume47
Issue number3
DOIs
Publication statusPublished - Sep 1986

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Keywords

  • Brain specific small RNA
  • Dot blot hybridization
  • Mouse genomic clone
  • Northern blot analysis
  • Nucleotide sequence analysis

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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