Isolation and sequence analysis of a cDNA and a related gene for cytochrome P450 proteins from Solanum chacoense

György Hutvágner, Endre Barta, Zsófia Bánfalvi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Inosine-containing degenerate PCR primers corresponding to the heme-binding domain of cytochrome P450 proteins have been synthesized and used for cloning cDNAs by the RT-PCR technique from Solanum chacoense. One clone in which the primer was immediately followed by sequences corresponding to the remaining part of the conserved domain was obtained. A leaf cDNA and a genomic library were constructed from S. chacoense. Clones homologous to the PCR fragment were isolated by plaque hybridization from both libraries (CYPs.ch-1 and CYPs.ch-2, respectively). Based on DNA sequence analysis, the selected clones are 87.6% identical and belong to the CYP71 family. The CYPs.ch genes are present in multiple copies in the S. chacoense as well as in the S. tuberosum genome with some polymorphisms. The CYPs.ch transcripts are slightly induced by methyl jasmonate and abscisic acid in S. chacoense foliage.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalGene
Volume188
Issue number2
DOIs
Publication statusPublished - Apr 1 1997

Keywords

  • Abscisic acid
  • CYP71 family
  • Gene expression
  • Methyl jasmonate
  • PCR-based cloning

ASJC Scopus subject areas

  • Genetics

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