Isolation and quantitative analysis of physalin d in the fruit and calyx of Physalis alkekengi L.

Eszter Laczkó-Zöld, P. Forgó, I. Zupkó, Esianu Sigrid, J. Hohmann

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Physalin D was isolated from the methanol extract of Physalis alkekengi L. fruits by combination of different chromatographic methods (CPC, TLC, HPLC). The structure was elucidated based on 1H and 13C NMR spectral analysis with the aid of 2D-correlation spectroscopy (1H, 1H-COSY, HSQC and HMBC) and comparison with literature data. The quantity of physalin D in mature and immature fruits and calyces was determined by RP-HPLC-UV method. Among the studied samples, immature calyx showed the highest content of physalin D (0.7880 ± 0.0612%), while mature calyx contained 4 times less amount (0.2028 ± 0.016%). The physalin D content of the fruit was much lower; immature fruits contained 0.0992 ± 0.0083% physalin D and mature fruits 0.0259 ± 0.0021%. The antiproliferative activity of the CHCl3 extract and its fractions was tested on three cancer cell lines (HeLa, MCF-7 and A431). The antiproliferative activity of physalin D is discussed with regard the published data.

Original languageEnglish
Pages (from-to)300-309
Number of pages10
JournalActa Biologica Hungarica
Volume68
Issue number3
DOIs
Publication statusPublished - Sep 1 2017

Fingerprint

Physalis
Fruits
quantitative analysis
Fruit
fruit
Chemical analysis
High Pressure Liquid Chromatography
spectral analysis
nuclear magnetic resonance
methanol
cancer
Spectrum analysis
spectroscopy
Methanol
physalin D
Spectrum Analysis
Cells
Nuclear magnetic resonance
Spectroscopy
Cell Line

Keywords

  • Cytotoxic activity
  • Physalin D
  • Physalis alkekengi
  • Solanaceae
  • Steroids

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

Isolation and quantitative analysis of physalin d in the fruit and calyx of Physalis alkekengi L. / Laczkó-Zöld, Eszter; Forgó, P.; Zupkó, I.; Sigrid, Esianu; Hohmann, J.

In: Acta Biologica Hungarica, Vol. 68, No. 3, 01.09.2017, p. 300-309.

Research output: Contribution to journalArticle

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