Isolation and identification of metabolites of 3H- and 14C- deramciclane by OPLC-digital autoradiography on-line sample collection and mass spectrometry

Imre Klebovich, Emil Mincsovics, József Szúnyog, Krisztina Ludányi, Tamás Karancsi, Kálmán Újszászy, Borbála Dalmadi Kiss, Károly Vékey

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A new simple and powerful technique, on-line sample collection after high-resolution step-wise gradient OPLC separation combined with digital autoradiography (DAR), has been used for isolation of plasma and urine metabolites of 3H- or 14C-radiolabeled deramciclane (a new anxiolytic compound). This highly efficient separation can be followed by different MS techniques (FAB-MS, FAB-MS-MS, HPLC-MS and HPLC-MS-MS) for determination of the structures of minor and major metabolites in different biological matrices. This combination of these methods resulted in a simple, sensitive, and efficient technique for metabolite research.

Original languageEnglish
Pages (from-to)394-399
Number of pages6
JournalJournal of Planar Chromatography - Modern TLC
Volume11
Issue number5
Publication statusPublished - Sep 1 1998

Keywords

  • Deramciclane (EGIS-3886)
  • Digital autoradiography (DAR)
  • Fast-atom bombardment (FAB- (Tandem) MS)
  • HPLC-MS- MS
  • Mass spectrometry (MS)
  • Metabolism
  • OPLC on-line sample collection

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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