Isolation and characterization of rabbit skeletal muscle protein phosphatase C-I and C-II

S. R. Silberman, M. Speth, R. Nemani, M. K. Ganapathi, V. Dombradi, H. Paris, E. Y. Lee

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Abstract

Previous studies have shown that phosphorylase phosphatase can be isolated from rabbit liver and bovine heart as a form of M(r) ~ 35,000 after an ethanol treatment of tissue extracts. This enzyme form was designated as protein phosphatase C. In the present study, reproducible methods for the isolation of two forms of protein phosphatase C from rabbit skeletal muscle to apparent homogeneity are described. Protein phosphatase C-I was obtained in yields of up to 20%, with specific activities toward phosphorylase a of 8,000-16,000 units/mg of protein. This enzyme represents the major phosphorylase phosphatase activity present in the ethanol-treated muscle extracts. The second enzyme, protein phosphatase C-II, had a much lower specific activity toward phosphorylase a (250-900 units/mg). Phosphatase C-I and phosphatase C-II had M(r) = 32,000 and 33,500, respectively, as determined by sodium dodecyl sulfate disc gel electrophoresis. The two enzymes displayed distinct enzymatic properties. Phosphatase C-II was associated with a more active alkaline phosphatase activity with a more active alkaline phosphatase activity toward p-nitrophenyl phosphate than was phosphatase C-I. Phosphatase C-II activities were activated by Mn2+, whereas phosphatase C-I was inhibited. Phosphatase C-I was inhibited by rabbit skeletal muscle inhibitor 2 while phosphatase C-II was not inhibited. Both enzymes dephosphorylated glycogen synthase and phosphorylase kinase, but displayed different specificities toward the α- and β-subunit phosphates of phosphorylase kinase. The amino acid compositions of the two proteins were similar. Peptide mapping of the two proteins showed that they are distinct proteins and do not have a precursor-proteolytic product relationship.

Original languageEnglish
Pages (from-to)2913-2922
Number of pages10
JournalJournal of Biological Chemistry
Volume259
Issue number5
Publication statusPublished - Jun 7 1984

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Silberman, S. R., Speth, M., Nemani, R., Ganapathi, M. K., Dombradi, V., Paris, H., & Lee, E. Y. (1984). Isolation and characterization of rabbit skeletal muscle protein phosphatase C-I and C-II. Journal of Biological Chemistry, 259(5), 2913-2922.