Isolation and characterization of Neosartorya fischeri antifungal protein (NFAP)

Laura Kovács, Máté Virágh, Miklós Takó, Tamás Papp, Csaba Vágvölgyi, László Galgóczy

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

A novel 6.6 kDa antifungal peptide (NFAP) from the culture supernatant of the mold, Neosartorya fischeri (anamorf: Aspergillus fischerianus), and its encoding gene were isolated in this study. NFAP is a small, basic and cysteine-rich protein consisting of 57 amino acid residues. It shows 37.9-50% homology to similar proteins described in literature from Aspergillus clavatus, Aspergillus giganteus, Aspergillus niger, and Penicillium chrysogenum. The in silico presumed tertiary structure of NFAP, e.g. the presence of five antiparallel β-sheet connected with filaments, and stabilized by three disulfide bridges, is very similar to those of the defensin-like molecules. NFAP exhibited growth inhibitory action against filamentous fungi in a dose-dependent manner, and maintained high antifungal activity within broad pH and temperature ranges. Furthermore, it exhibited relevant resistance to proteolysis. All these characteristics make NFAP a promising candidate for further in vitro and in vivo investigations aiming at the development of new antifungal compounds.

Original languageEnglish
Pages (from-to)1724-1731
Number of pages8
JournalPeptides
Volume32
Issue number8
DOIs
Publication statusPublished - Aug 2011

Fingerprint

Neosartorya
Aspergillus
Proteins
Fungi
Penicillium chrysogenum
Proteolysis
Defensins
Gene encoding
Aspergillus niger
Disulfides
Computer Simulation
Cysteine
Amino Acids
Peptides
Molecules
Temperature

Keywords

  • Antifungal activity
  • Antifungal susceptibility
  • Defensin-like protein
  • Filamentous fungi
  • Protein isolation

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Isolation and characterization of Neosartorya fischeri antifungal protein (NFAP). / Kovács, Laura; Virágh, Máté; Takó, Miklós; Papp, Tamás; Vágvölgyi, Csaba; Galgóczy, László.

In: Peptides, Vol. 32, No. 8, 08.2011, p. 1724-1731.

Research output: Contribution to journalArticle

Kovács, Laura ; Virágh, Máté ; Takó, Miklós ; Papp, Tamás ; Vágvölgyi, Csaba ; Galgóczy, László. / Isolation and characterization of Neosartorya fischeri antifungal protein (NFAP). In: Peptides. 2011 ; Vol. 32, No. 8. pp. 1724-1731.
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