Isolation and characterization of a novel n-alkane-degrading strain, Acinetobacter haemolyticus AR-46

Zoltán Bihari, Aladár Pettkó-Szandtner, Gyula Csanádi, Margit Balázs, Péter Bartos, Péter Kesseru, I. Kiss, Imre Mécs

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Strain AR-46, isolated and identified as Acinetobacter haemolyticus, evolutionally distant from the known hydrocarbon-degrading Acinetobacter spp., proved to have excellent long-chain n-alkane-degrading ability. This is the first detailed report on an n-alkane-utilizing strain belonging to this species. The preferred substrate is n-hexadecane, with an optimal temperature of 37°C under aerobic conditions. Five complete and two partial open reading frames were sequenced and correlated with the early steps of monoterminal oxidation-initiated n-alkane mineralization. The encoded protein sequences and the arrangement of these genes displayed high similarity to those found in Acinetobacter sp. M-1, but AR-46 seemed to have only one alkane hydroxylase gene, with a completely different induction profile. Unique behaviour was also observed in n-alkane bioavailability. Substrate uptake occurred through the hydrophobic surface of n-alkane droplet-adhered cells possessing long, thick fimbriae, which were presumed to play a major role in n-alkane solubilization. A majority of the cells was in detached form, with thick, but short fimbriae. These free cells were permanently hydrophilic, unlike the cells of other Acinetobacter strains.

Original languageEnglish
Pages (from-to)285-295
Number of pages11
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume62
Issue number3-4
Publication statusPublished - Mar 2007

Fingerprint

Acinetobacter haemolyticus
Acinetobacter
Alkanes
alkanes
fimbriae
Cytochrome P-450 CYP4A
Genes
cells
Gene Order
hexadecane
Substrates
Hydrocarbons
aerobic conditions
Biological Availability
Open Reading Frames
solubilization
droplets
open reading frames
hydrocarbons
bioavailability

Keywords

  • alkM induction
  • Direct adherence
  • n-alkane

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Medicine(all)

Cite this

Bihari, Z., Pettkó-Szandtner, A., Csanádi, G., Balázs, M., Bartos, P., Kesseru, P., ... Mécs, I. (2007). Isolation and characterization of a novel n-alkane-degrading strain, Acinetobacter haemolyticus AR-46. Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 62(3-4), 285-295.

Isolation and characterization of a novel n-alkane-degrading strain, Acinetobacter haemolyticus AR-46. / Bihari, Zoltán; Pettkó-Szandtner, Aladár; Csanádi, Gyula; Balázs, Margit; Bartos, Péter; Kesseru, Péter; Kiss, I.; Mécs, Imre.

In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences, Vol. 62, No. 3-4, 03.2007, p. 285-295.

Research output: Contribution to journalArticle

Bihari, Z, Pettkó-Szandtner, A, Csanádi, G, Balázs, M, Bartos, P, Kesseru, P, Kiss, I & Mécs, I 2007, 'Isolation and characterization of a novel n-alkane-degrading strain, Acinetobacter haemolyticus AR-46', Zeitschrift fur Naturforschung - Section C Journal of Biosciences, vol. 62, no. 3-4, pp. 285-295.
Bihari, Zoltán ; Pettkó-Szandtner, Aladár ; Csanádi, Gyula ; Balázs, Margit ; Bartos, Péter ; Kesseru, Péter ; Kiss, I. ; Mécs, Imre. / Isolation and characterization of a novel n-alkane-degrading strain, Acinetobacter haemolyticus AR-46. In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences. 2007 ; Vol. 62, No. 3-4. pp. 285-295.
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