Isolation and Characterization of a Murine Spontaneous High-Grade Follicular Lymphoma with Restricted In Vivo Spreading – a Model for Lymphatic Metastasis Via the Mesentery

Dóra Vojkovics, Zoltán Kellermayer, Diána Heidt, Martina Mihalj, Béla Kajtár, Dávid Ernszt, Tamás Kovács, P. Németh, P. Balogh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Spontaneous or induced malignant lymphomas in mice are valuable tools for studying human lymphoproliferative diseases, including the mechanism of migration between peripheral lymphoid organs and positioning within distinct tissue compartments. Here we report the isolation and characterization of a novel spontaneous lymphoma from BALB/c mice showing restricted tissue distribution and metastasis. The lymphoma cells display CD19, B220, MHC II, surface IgG2a/kappa chain with VH7183 rearrangement of the IgH gene, indicating their B-cell origin. Serial intraperitoneal injection of primary tumor into both BALB/c and RAG-1-deficient hosts led to the successful propagation of lymphoma. Despite the cytological characteristics of high-grade follicular B-cell lymphoma, the tumor cells (denoted as Bc-DLFL.1) showed significantly lesser spreading to extraabdominal locations upon intraperitoneal passage compared to splenic and mesenteric lymph node expansion. In mesenteric lymph nodes the high endothelial venules contained only few tumor cells, while the lymphatic vessels were almost completely filled with lymphoma cells. Similarly, the LYVE-1-positive lymphatic capillaries within the mesentery were packed with lymphoma cells. These findings suggest that Bc-DLFL.1 cells likely propagate primarily via the lymphatic circulation within the mesentery, therefore this tumor may offer an in vivo model to investigate the tumor cell migration via the lymphatic circulation from the peritoneal cavity.

Original languageEnglish
Pages (from-to)421-430
Number of pages10
JournalPathology and Oncology Research
Volume22
Issue number2
DOIs
Publication statusPublished - Apr 1 2016

Fingerprint

Lymphatic Metastasis
Follicular Lymphoma
Mesentery
Non-Hodgkin's Lymphoma
Lymphoma
Lymphatic Vessels
Neoplasms
Lymph Nodes
Venules
Gene Rearrangement
Peritoneal Cavity
B-Cell Lymphoma
Tissue Distribution
Intraperitoneal Injections
Cell Movement
B-Lymphocytes
Neoplasm Metastasis

Keywords

  • Follicular lymphoma
  • Lymphatics
  • Model
  • Mouse

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Isolation and Characterization of a Murine Spontaneous High-Grade Follicular Lymphoma with Restricted In Vivo Spreading – a Model for Lymphatic Metastasis Via the Mesentery. / Vojkovics, Dóra; Kellermayer, Zoltán; Heidt, Diána; Mihalj, Martina; Kajtár, Béla; Ernszt, Dávid; Kovács, Tamás; Németh, P.; Balogh, P.

In: Pathology and Oncology Research, Vol. 22, No. 2, 01.04.2016, p. 421-430.

Research output: Contribution to journalArticle

Vojkovics, Dóra ; Kellermayer, Zoltán ; Heidt, Diána ; Mihalj, Martina ; Kajtár, Béla ; Ernszt, Dávid ; Kovács, Tamás ; Németh, P. ; Balogh, P. / Isolation and Characterization of a Murine Spontaneous High-Grade Follicular Lymphoma with Restricted In Vivo Spreading – a Model for Lymphatic Metastasis Via the Mesentery. In: Pathology and Oncology Research. 2016 ; Vol. 22, No. 2. pp. 421-430.
@article{56e648dfa088416abdd1d0a9368b5727,
title = "Isolation and Characterization of a Murine Spontaneous High-Grade Follicular Lymphoma with Restricted In Vivo Spreading – a Model for Lymphatic Metastasis Via the Mesentery",
abstract = "Spontaneous or induced malignant lymphomas in mice are valuable tools for studying human lymphoproliferative diseases, including the mechanism of migration between peripheral lymphoid organs and positioning within distinct tissue compartments. Here we report the isolation and characterization of a novel spontaneous lymphoma from BALB/c mice showing restricted tissue distribution and metastasis. The lymphoma cells display CD19, B220, MHC II, surface IgG2a/kappa chain with VH7183 rearrangement of the IgH gene, indicating their B-cell origin. Serial intraperitoneal injection of primary tumor into both BALB/c and RAG-1-deficient hosts led to the successful propagation of lymphoma. Despite the cytological characteristics of high-grade follicular B-cell lymphoma, the tumor cells (denoted as Bc-DLFL.1) showed significantly lesser spreading to extraabdominal locations upon intraperitoneal passage compared to splenic and mesenteric lymph node expansion. In mesenteric lymph nodes the high endothelial venules contained only few tumor cells, while the lymphatic vessels were almost completely filled with lymphoma cells. Similarly, the LYVE-1-positive lymphatic capillaries within the mesentery were packed with lymphoma cells. These findings suggest that Bc-DLFL.1 cells likely propagate primarily via the lymphatic circulation within the mesentery, therefore this tumor may offer an in vivo model to investigate the tumor cell migration via the lymphatic circulation from the peritoneal cavity.",
keywords = "Follicular lymphoma, Lymphatics, Model, Mouse",
author = "D{\'o}ra Vojkovics and Zolt{\'a}n Kellermayer and Di{\'a}na Heidt and Martina Mihalj and B{\'e}la Kajt{\'a}r and D{\'a}vid Ernszt and Tam{\'a}s Kov{\'a}cs and P. N{\'e}meth and P. Balogh",
year = "2016",
month = "4",
day = "1",
doi = "10.1007/s12253-015-0025-6",
language = "English",
volume = "22",
pages = "421--430",
journal = "Pathology and Oncology Research",
issn = "1219-4956",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Isolation and Characterization of a Murine Spontaneous High-Grade Follicular Lymphoma with Restricted In Vivo Spreading – a Model for Lymphatic Metastasis Via the Mesentery

AU - Vojkovics, Dóra

AU - Kellermayer, Zoltán

AU - Heidt, Diána

AU - Mihalj, Martina

AU - Kajtár, Béla

AU - Ernszt, Dávid

AU - Kovács, Tamás

AU - Németh, P.

AU - Balogh, P.

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Spontaneous or induced malignant lymphomas in mice are valuable tools for studying human lymphoproliferative diseases, including the mechanism of migration between peripheral lymphoid organs and positioning within distinct tissue compartments. Here we report the isolation and characterization of a novel spontaneous lymphoma from BALB/c mice showing restricted tissue distribution and metastasis. The lymphoma cells display CD19, B220, MHC II, surface IgG2a/kappa chain with VH7183 rearrangement of the IgH gene, indicating their B-cell origin. Serial intraperitoneal injection of primary tumor into both BALB/c and RAG-1-deficient hosts led to the successful propagation of lymphoma. Despite the cytological characteristics of high-grade follicular B-cell lymphoma, the tumor cells (denoted as Bc-DLFL.1) showed significantly lesser spreading to extraabdominal locations upon intraperitoneal passage compared to splenic and mesenteric lymph node expansion. In mesenteric lymph nodes the high endothelial venules contained only few tumor cells, while the lymphatic vessels were almost completely filled with lymphoma cells. Similarly, the LYVE-1-positive lymphatic capillaries within the mesentery were packed with lymphoma cells. These findings suggest that Bc-DLFL.1 cells likely propagate primarily via the lymphatic circulation within the mesentery, therefore this tumor may offer an in vivo model to investigate the tumor cell migration via the lymphatic circulation from the peritoneal cavity.

AB - Spontaneous or induced malignant lymphomas in mice are valuable tools for studying human lymphoproliferative diseases, including the mechanism of migration between peripheral lymphoid organs and positioning within distinct tissue compartments. Here we report the isolation and characterization of a novel spontaneous lymphoma from BALB/c mice showing restricted tissue distribution and metastasis. The lymphoma cells display CD19, B220, MHC II, surface IgG2a/kappa chain with VH7183 rearrangement of the IgH gene, indicating their B-cell origin. Serial intraperitoneal injection of primary tumor into both BALB/c and RAG-1-deficient hosts led to the successful propagation of lymphoma. Despite the cytological characteristics of high-grade follicular B-cell lymphoma, the tumor cells (denoted as Bc-DLFL.1) showed significantly lesser spreading to extraabdominal locations upon intraperitoneal passage compared to splenic and mesenteric lymph node expansion. In mesenteric lymph nodes the high endothelial venules contained only few tumor cells, while the lymphatic vessels were almost completely filled with lymphoma cells. Similarly, the LYVE-1-positive lymphatic capillaries within the mesentery were packed with lymphoma cells. These findings suggest that Bc-DLFL.1 cells likely propagate primarily via the lymphatic circulation within the mesentery, therefore this tumor may offer an in vivo model to investigate the tumor cell migration via the lymphatic circulation from the peritoneal cavity.

KW - Follicular lymphoma

KW - Lymphatics

KW - Model

KW - Mouse

UR - http://www.scopus.com/inward/record.url?scp=84961197147&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84961197147&partnerID=8YFLogxK

U2 - 10.1007/s12253-015-0025-6

DO - 10.1007/s12253-015-0025-6

M3 - Article

C2 - 26584567

AN - SCOPUS:84961197147

VL - 22

SP - 421

EP - 430

JO - Pathology and Oncology Research

JF - Pathology and Oncology Research

SN - 1219-4956

IS - 2

ER -