Abstract
Spontaneous or induced malignant lymphomas in mice are valuable tools for studying human lymphoproliferative diseases, including the mechanism of migration between peripheral lymphoid organs and positioning within distinct tissue compartments. Here we report the isolation and characterization of a novel spontaneous lymphoma from BALB/c mice showing restricted tissue distribution and metastasis. The lymphoma cells display CD19, B220, MHC II, surface IgG2a/kappa chain with VH7183 rearrangement of the IgH gene, indicating their B-cell origin. Serial intraperitoneal injection of primary tumor into both BALB/c and RAG-1-deficient hosts led to the successful propagation of lymphoma. Despite the cytological characteristics of high-grade follicular B-cell lymphoma, the tumor cells (denoted as Bc-DLFL.1) showed significantly lesser spreading to extraabdominal locations upon intraperitoneal passage compared to splenic and mesenteric lymph node expansion. In mesenteric lymph nodes the high endothelial venules contained only few tumor cells, while the lymphatic vessels were almost completely filled with lymphoma cells. Similarly, the LYVE-1-positive lymphatic capillaries within the mesentery were packed with lymphoma cells. These findings suggest that Bc-DLFL.1 cells likely propagate primarily via the lymphatic circulation within the mesentery, therefore this tumor may offer an in vivo model to investigate the tumor cell migration via the lymphatic circulation from the peritoneal cavity.
| Original language | English |
|---|---|
| Pages (from-to) | 421-430 |
| Number of pages | 10 |
| Journal | Pathology and Oncology Research |
| Volume | 22 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Apr 1 2016 |
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Keywords
- Follicular lymphoma
- Lymphatics
- Model
- Mouse
ASJC Scopus subject areas
- Cancer Research
- Oncology
- Pathology and Forensic Medicine
Cite this
Isolation and Characterization of a Murine Spontaneous High-Grade Follicular Lymphoma with Restricted In Vivo Spreading – a Model for Lymphatic Metastasis Via the Mesentery. / Vojkovics, Dóra; Kellermayer, Zoltán; Heidt, Diána; Mihalj, Martina; Kajtár, Béla; Ernszt, Dávid; Kovács, Tamás; Németh, P.; Balogh, P.
In: Pathology and Oncology Research, Vol. 22, No. 2, 01.04.2016, p. 421-430.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and Characterization of a Murine Spontaneous High-Grade Follicular Lymphoma with Restricted In Vivo Spreading – a Model for Lymphatic Metastasis Via the Mesentery
AU - Vojkovics, Dóra
AU - Kellermayer, Zoltán
AU - Heidt, Diána
AU - Mihalj, Martina
AU - Kajtár, Béla
AU - Ernszt, Dávid
AU - Kovács, Tamás
AU - Németh, P.
AU - Balogh, P.
PY - 2016/4/1
Y1 - 2016/4/1
N2 - Spontaneous or induced malignant lymphomas in mice are valuable tools for studying human lymphoproliferative diseases, including the mechanism of migration between peripheral lymphoid organs and positioning within distinct tissue compartments. Here we report the isolation and characterization of a novel spontaneous lymphoma from BALB/c mice showing restricted tissue distribution and metastasis. The lymphoma cells display CD19, B220, MHC II, surface IgG2a/kappa chain with VH7183 rearrangement of the IgH gene, indicating their B-cell origin. Serial intraperitoneal injection of primary tumor into both BALB/c and RAG-1-deficient hosts led to the successful propagation of lymphoma. Despite the cytological characteristics of high-grade follicular B-cell lymphoma, the tumor cells (denoted as Bc-DLFL.1) showed significantly lesser spreading to extraabdominal locations upon intraperitoneal passage compared to splenic and mesenteric lymph node expansion. In mesenteric lymph nodes the high endothelial venules contained only few tumor cells, while the lymphatic vessels were almost completely filled with lymphoma cells. Similarly, the LYVE-1-positive lymphatic capillaries within the mesentery were packed with lymphoma cells. These findings suggest that Bc-DLFL.1 cells likely propagate primarily via the lymphatic circulation within the mesentery, therefore this tumor may offer an in vivo model to investigate the tumor cell migration via the lymphatic circulation from the peritoneal cavity.
AB - Spontaneous or induced malignant lymphomas in mice are valuable tools for studying human lymphoproliferative diseases, including the mechanism of migration between peripheral lymphoid organs and positioning within distinct tissue compartments. Here we report the isolation and characterization of a novel spontaneous lymphoma from BALB/c mice showing restricted tissue distribution and metastasis. The lymphoma cells display CD19, B220, MHC II, surface IgG2a/kappa chain with VH7183 rearrangement of the IgH gene, indicating their B-cell origin. Serial intraperitoneal injection of primary tumor into both BALB/c and RAG-1-deficient hosts led to the successful propagation of lymphoma. Despite the cytological characteristics of high-grade follicular B-cell lymphoma, the tumor cells (denoted as Bc-DLFL.1) showed significantly lesser spreading to extraabdominal locations upon intraperitoneal passage compared to splenic and mesenteric lymph node expansion. In mesenteric lymph nodes the high endothelial venules contained only few tumor cells, while the lymphatic vessels were almost completely filled with lymphoma cells. Similarly, the LYVE-1-positive lymphatic capillaries within the mesentery were packed with lymphoma cells. These findings suggest that Bc-DLFL.1 cells likely propagate primarily via the lymphatic circulation within the mesentery, therefore this tumor may offer an in vivo model to investigate the tumor cell migration via the lymphatic circulation from the peritoneal cavity.
KW - Follicular lymphoma
KW - Lymphatics
KW - Model
KW - Mouse
UR - http://www.scopus.com/inward/record.url?scp=84961197147&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84961197147&partnerID=8YFLogxK
U2 - 10.1007/s12253-015-0025-6
DO - 10.1007/s12253-015-0025-6
M3 - Article
C2 - 26584567
AN - SCOPUS:84961197147
VL - 22
SP - 421
EP - 430
JO - Pathology and Oncology Research
JF - Pathology and Oncology Research
SN - 1219-4956
IS - 2
ER -