Isolation and characterisation of 15 microsatellite loci from Lethrus apterus (Coleoptera: Geotrupidae)

Rita Rácz, Judit Bereczki, Gábor Sramkó, A. Kosztolányi, János P. Tóth, Szilárd Póliska, Attila Horváth, E. Barta, Z. Barta

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Fifteen new microsatellite markers for the beetle Lethrus apterus were developed and tested in 45 specimens from the North Hungarian Mountains. Fourteen of the markers developed were polymorphic, and the number of alleles per locus ranged from two to nine. The observed and expected heterozygosities of the polymorphic markers ranged from 0.178 to 0.578, and 0.201 to 0.698, respectively. One locus showed significant deviation from the Hardy-Weinberg equilibrium, probably due to null alleles. The primers were tested on four other Lethrus species (L. bituberculatus, L. scoparius, L. strymonensis and L. perun) and six other coleopteran species (Copris hispanus, Geotrupes stercorarius, Melolontha melolontha, Onthophagus taurus, Oryctes nasicornis and Protaetia affinis). Thirteen loci showed cross-amplification in the Lethrus species and only three loci could be amplified in some of the six other coleopteran species. The developed markers will be valuable in investigating the population genetic structure, behaviour and reproductive biology of L. apterus.

Original languageEnglish
Pages (from-to)45-50
Number of pages6
JournalAnnales Zoologici Fennici
Volume52
Issue number1-2
DOIs
Publication statusPublished - Apr 1 2015

Fingerprint

Geotrupidae
microsatellite repeats
Coleoptera
loci
Protaetia
allele
Geotrupes
Copris
Oryctes (Coleoptera)
Onthophagus taurus
Melolontha melolontha
null alleles
reproductive biology
heterozygosity
genetic structure
population genetics
amplification
beetle
mountains
alleles

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Nature and Landscape Conservation

Cite this

Isolation and characterisation of 15 microsatellite loci from Lethrus apterus (Coleoptera : Geotrupidae). / Rácz, Rita; Bereczki, Judit; Sramkó, Gábor; Kosztolányi, A.; Tóth, János P.; Póliska, Szilárd; Horváth, Attila; Barta, E.; Barta, Z.

In: Annales Zoologici Fennici, Vol. 52, No. 1-2, 01.04.2015, p. 45-50.

Research output: Contribution to journalArticle

Rácz, Rita ; Bereczki, Judit ; Sramkó, Gábor ; Kosztolányi, A. ; Tóth, János P. ; Póliska, Szilárd ; Horváth, Attila ; Barta, E. ; Barta, Z. / Isolation and characterisation of 15 microsatellite loci from Lethrus apterus (Coleoptera : Geotrupidae). In: Annales Zoologici Fennici. 2015 ; Vol. 52, No. 1-2. pp. 45-50.
@article{5d263d0c7bf34ad0a07a7ff9e76d1158,
title = "Isolation and characterisation of 15 microsatellite loci from Lethrus apterus (Coleoptera: Geotrupidae)",
abstract = "Fifteen new microsatellite markers for the beetle Lethrus apterus were developed and tested in 45 specimens from the North Hungarian Mountains. Fourteen of the markers developed were polymorphic, and the number of alleles per locus ranged from two to nine. The observed and expected heterozygosities of the polymorphic markers ranged from 0.178 to 0.578, and 0.201 to 0.698, respectively. One locus showed significant deviation from the Hardy-Weinberg equilibrium, probably due to null alleles. The primers were tested on four other Lethrus species (L. bituberculatus, L. scoparius, L. strymonensis and L. perun) and six other coleopteran species (Copris hispanus, Geotrupes stercorarius, Melolontha melolontha, Onthophagus taurus, Oryctes nasicornis and Protaetia affinis). Thirteen loci showed cross-amplification in the Lethrus species and only three loci could be amplified in some of the six other coleopteran species. The developed markers will be valuable in investigating the population genetic structure, behaviour and reproductive biology of L. apterus.",
author = "Rita R{\'a}cz and Judit Bereczki and G{\'a}bor Sramk{\'o} and A. Kosztol{\'a}nyi and T{\'o}th, {J{\'a}nos P.} and Szil{\'a}rd P{\'o}liska and Attila Horv{\'a}th and E. Barta and Z. Barta",
year = "2015",
month = "4",
day = "1",
doi = "10.5735/086.052.0204",
language = "English",
volume = "52",
pages = "45--50",
journal = "Annales Zoologici Fennici",
issn = "0003-455X",
publisher = "Finnish Zoological and Botanical Publishing Board",
number = "1-2",

}

TY - JOUR

T1 - Isolation and characterisation of 15 microsatellite loci from Lethrus apterus (Coleoptera

T2 - Geotrupidae)

AU - Rácz, Rita

AU - Bereczki, Judit

AU - Sramkó, Gábor

AU - Kosztolányi, A.

AU - Tóth, János P.

AU - Póliska, Szilárd

AU - Horváth, Attila

AU - Barta, E.

AU - Barta, Z.

PY - 2015/4/1

Y1 - 2015/4/1

N2 - Fifteen new microsatellite markers for the beetle Lethrus apterus were developed and tested in 45 specimens from the North Hungarian Mountains. Fourteen of the markers developed were polymorphic, and the number of alleles per locus ranged from two to nine. The observed and expected heterozygosities of the polymorphic markers ranged from 0.178 to 0.578, and 0.201 to 0.698, respectively. One locus showed significant deviation from the Hardy-Weinberg equilibrium, probably due to null alleles. The primers were tested on four other Lethrus species (L. bituberculatus, L. scoparius, L. strymonensis and L. perun) and six other coleopteran species (Copris hispanus, Geotrupes stercorarius, Melolontha melolontha, Onthophagus taurus, Oryctes nasicornis and Protaetia affinis). Thirteen loci showed cross-amplification in the Lethrus species and only three loci could be amplified in some of the six other coleopteran species. The developed markers will be valuable in investigating the population genetic structure, behaviour and reproductive biology of L. apterus.

AB - Fifteen new microsatellite markers for the beetle Lethrus apterus were developed and tested in 45 specimens from the North Hungarian Mountains. Fourteen of the markers developed were polymorphic, and the number of alleles per locus ranged from two to nine. The observed and expected heterozygosities of the polymorphic markers ranged from 0.178 to 0.578, and 0.201 to 0.698, respectively. One locus showed significant deviation from the Hardy-Weinberg equilibrium, probably due to null alleles. The primers were tested on four other Lethrus species (L. bituberculatus, L. scoparius, L. strymonensis and L. perun) and six other coleopteran species (Copris hispanus, Geotrupes stercorarius, Melolontha melolontha, Onthophagus taurus, Oryctes nasicornis and Protaetia affinis). Thirteen loci showed cross-amplification in the Lethrus species and only three loci could be amplified in some of the six other coleopteran species. The developed markers will be valuable in investigating the population genetic structure, behaviour and reproductive biology of L. apterus.

UR - http://www.scopus.com/inward/record.url?scp=84927667317&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84927667317&partnerID=8YFLogxK

U2 - 10.5735/086.052.0204

DO - 10.5735/086.052.0204

M3 - Article

AN - SCOPUS:84927667317

VL - 52

SP - 45

EP - 50

JO - Annales Zoologici Fennici

JF - Annales Zoologici Fennici

SN - 0003-455X

IS - 1-2

ER -