Isohydric and anisohydric strategies of wheat genotypes under osmotic stress: Biosynthesis and function of ABA in stress responses

Ágnes Gallé, J. Csiszár, Dániel Benyó, Gábor Laskay, Tünde Leviczky, L. Erdei, I. Tari

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Changes in water potential (ψw), stomatal conductance, abscisic acid (ABA) accumulation, expression of the major genes involved in ABA biosynthesis, activities of abscisic aldehyde oxidase (AO, EC 1.2.3.1) and antioxidant enzymes were studied in two wheat cultivars with contrasting acclimation strategies subjected to medium strength osmotic stress (-0.976MPa) induced by polyethylene glycol (PEG 6000). Because the biosynthetic pathway of ABA involves multiple gene products, the aim of this study was to unravel how these genes are regulated in isohydric and anisohydric wheat genotypes. In the root tissues of the isohydric cultivar, Triticum aestivum cv. Kobomugi, osmotic stress increased the transcript levels of 9-cis-epoxycarotenoid dioxygenase (NCED) gene, controlling the rate limiting step of ABA biosynthesis. Moreover, this cultivar exhibited a higher basal activity and a higher induction of aldehyde oxidase isoenzymes (AAO2-AAO3), responsible for converting ABAldehyde to ABA. It was found that the fast activation of the ABA biosynthesis in the roots generated an enhanced ABA pool in the shoot, which brought about a faster closure of the stomata upon increasing osmotic stress and, as a result, the plants could maintain ψw in the tissues close to the control level. In contrast, the anisohydric genotype, cv. GK Öthalom, exhibited a moderate induction of ABA biosynthesis in the roots, leading to the maintenance but no increase in the concentration of ABA on the basis of tissue water content in the leaves. Due to the slower response of their stomata to water deficit, the tissues of cv. GK Öthalom have to acclimate to much more negative water potentials during increasing osmotic stress. A decreased activity of superoxide dismutase (SOD) was found in the leaves and roots of both cultivars exposed to osmotic stress, but in the roots elevated activities of catalase (CAT), peroxidase (POX), glutathione reductase (GR) and glutathione transferase (GST) were detected in the isohydric cultivar, suggesting that this genotype was more successful in the elimination of reactive oxygen species caused by the stress conditions.

Original languageEnglish
Pages (from-to)1389-1399
Number of pages11
JournalJournal of Plant Physiology
Volume170
Issue number16
DOIs
Publication statusPublished - Nov 1 2013

Fingerprint

Abscisic Acid
Osmotic Pressure
osmotic stress
Triticum
abscisic acid
stress response
Genotype
biosynthesis
wheat
genotype
cultivars
9-cis-epoxy-carotenoid dioxygenase
Water
stomata
water potential
aldehydes
Aldehyde Oxidase
Genes
genes
Glutathione Reductase

Keywords

  • Abscisic acid
  • Anisohydric strategy
  • Isohydric strategy
  • Osmotic stress
  • Triticum aestivum

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Agronomy and Crop Science

Cite this

Isohydric and anisohydric strategies of wheat genotypes under osmotic stress : Biosynthesis and function of ABA in stress responses. / Gallé, Ágnes; Csiszár, J.; Benyó, Dániel; Laskay, Gábor; Leviczky, Tünde; Erdei, L.; Tari, I.

In: Journal of Plant Physiology, Vol. 170, No. 16, 01.11.2013, p. 1389-1399.

Research output: Contribution to journalArticle

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