Isoflurane causes neocortical but not hippocampal-dependent memory impairment in mice

A. R. Fidalgo, M. Cibelli, J. P M White, I. Nagy, Y. Wan, D. Ma

Research output: Contribution to journalArticle

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Abstract

Background The aim of this study is to investigate the effect of general anaesthesia induced by isoflurane with buprenorphine on hippocampus-dependent and neocortex-dependent memory, respectively, in mice, and in addition, to compare the effects of such anaesthesia on these memory processes with the effects induced by lipopolysaccharide (LPS) administration on the same memory processes. Methods To assess hippocampus-dependent memory, isoflurane (for 15min) after buprenorphine injection, or LPS 100μg/kg (intraperitoneally) was administered 24h before or after fear conditioning. The effect of these treatments on hippocampus-dependent memory was assessed using contextual fear-conditioning tasks at day 4. To assess neocortex-dependent memory, isoflurane anaesthesia or LPS was given 72h after contextual fear conditioning. Neocortex-dependent memory assessment was performed at day 32. Results Unlike LPS injection, isoflurane with buprenorphine-induced anaesthesia does not impair freezing responses in hippocampus-dependent fear-conditioning memory tasks. On anterograde amnesia assessment: 49.67±6.87% for the anaesthesia group and 54.5±4.12% for the control group. On retrograde amnesia assessment: 47.16±8.71% for the anaesthesia group and 54.5±4.12% for control group; P>0.05. Thus, neither isoflurane nor buprenorphine impair hippocampus-dependent memory. However, on the neocortex-dependent memory task, both isoflurane-induced anaesthesia and LPS-induced inflammation result in reduced freezing responses: 62.13±5.80% for the anaesthesia group, 74.63±5.69% for the LPS group, and 81.75±3.26% for the control group; P

Original languageEnglish
Pages (from-to)1052-1057
Number of pages6
JournalActa Anaesthesiologica Scandinavica
Volume56
Issue number8
DOIs
Publication statusPublished - Sep 2012

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Isoflurane
Anesthesia
Lipopolysaccharides
Buprenorphine
Neocortex
Hippocampus
Fear
Control Groups
Freezing
Anterograde Amnesia
Retrograde Amnesia
Injections
General Anesthesia
Inflammation
Conditioning (Psychology)

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

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Isoflurane causes neocortical but not hippocampal-dependent memory impairment in mice. / Fidalgo, A. R.; Cibelli, M.; White, J. P M; Nagy, I.; Wan, Y.; Ma, D.

In: Acta Anaesthesiologica Scandinavica, Vol. 56, No. 8, 09.2012, p. 1052-1057.

Research output: Contribution to journalArticle

Fidalgo, A. R. ; Cibelli, M. ; White, J. P M ; Nagy, I. ; Wan, Y. ; Ma, D. / Isoflurane causes neocortical but not hippocampal-dependent memory impairment in mice. In: Acta Anaesthesiologica Scandinavica. 2012 ; Vol. 56, No. 8. pp. 1052-1057.
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abstract = "Background The aim of this study is to investigate the effect of general anaesthesia induced by isoflurane with buprenorphine on hippocampus-dependent and neocortex-dependent memory, respectively, in mice, and in addition, to compare the effects of such anaesthesia on these memory processes with the effects induced by lipopolysaccharide (LPS) administration on the same memory processes. Methods To assess hippocampus-dependent memory, isoflurane (for 15min) after buprenorphine injection, or LPS 100μg/kg (intraperitoneally) was administered 24h before or after fear conditioning. The effect of these treatments on hippocampus-dependent memory was assessed using contextual fear-conditioning tasks at day 4. To assess neocortex-dependent memory, isoflurane anaesthesia or LPS was given 72h after contextual fear conditioning. Neocortex-dependent memory assessment was performed at day 32. Results Unlike LPS injection, isoflurane with buprenorphine-induced anaesthesia does not impair freezing responses in hippocampus-dependent fear-conditioning memory tasks. On anterograde amnesia assessment: 49.67±6.87{\%} for the anaesthesia group and 54.5±4.12{\%} for the control group. On retrograde amnesia assessment: 47.16±8.71{\%} for the anaesthesia group and 54.5±4.12{\%} for control group; P>0.05. Thus, neither isoflurane nor buprenorphine impair hippocampus-dependent memory. However, on the neocortex-dependent memory task, both isoflurane-induced anaesthesia and LPS-induced inflammation result in reduced freezing responses: 62.13±5.80{\%} for the anaesthesia group, 74.63±5.69{\%} for the LPS group, and 81.75±3.26{\%} for the control group; P",
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AB - Background The aim of this study is to investigate the effect of general anaesthesia induced by isoflurane with buprenorphine on hippocampus-dependent and neocortex-dependent memory, respectively, in mice, and in addition, to compare the effects of such anaesthesia on these memory processes with the effects induced by lipopolysaccharide (LPS) administration on the same memory processes. Methods To assess hippocampus-dependent memory, isoflurane (for 15min) after buprenorphine injection, or LPS 100μg/kg (intraperitoneally) was administered 24h before or after fear conditioning. The effect of these treatments on hippocampus-dependent memory was assessed using contextual fear-conditioning tasks at day 4. To assess neocortex-dependent memory, isoflurane anaesthesia or LPS was given 72h after contextual fear conditioning. Neocortex-dependent memory assessment was performed at day 32. Results Unlike LPS injection, isoflurane with buprenorphine-induced anaesthesia does not impair freezing responses in hippocampus-dependent fear-conditioning memory tasks. On anterograde amnesia assessment: 49.67±6.87% for the anaesthesia group and 54.5±4.12% for the control group. On retrograde amnesia assessment: 47.16±8.71% for the anaesthesia group and 54.5±4.12% for control group; P>0.05. Thus, neither isoflurane nor buprenorphine impair hippocampus-dependent memory. However, on the neocortex-dependent memory task, both isoflurane-induced anaesthesia and LPS-induced inflammation result in reduced freezing responses: 62.13±5.80% for the anaesthesia group, 74.63±5.69% for the LPS group, and 81.75±3.26% for the control group; P

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