Isoenzyme and microsatellite analysis of Vitis vinifera L. varieties from the Hungarian grape germplasm

G. Jahnke, J. Májer, A. Lakatos, J. Györffyné Molnár, E. Deák, E. Stefanovits-Banyai, P. Varga

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The aim of our work was to investigate the genetic diversity of grapevine with biochemical and molecular markers (isoenzyme and SSR). The isoenzyme patterns of 4 enzyme systems (catechol-oxidase, glutamate-oxalacetate-transaminase, acid phosphatase and peroxidase) and the microsatellite profile in 6 loci (VVS2, VVS16, VVMD7, VMC4A1, VMC4G6, VrZag79) of 48 grapevine varieties were analysed. The results with CO, GOT, AcP and PER enzymes were reproducible and the zymograms obtained from the woody stems were independent from the time of sampling during the dormant period of the grape. Based on the isoenzyme patterns of these 4 enzymes most of the investigated varieties (40/48) were identified. A correlation was found between the isoenzyme patterns and the classification to convarietas of the varieties. It was established, that while the varieties of the convarietas pontica differed from those of the convarietas orientalis and occidentalis, the two latter groups could have not been differentiated from each other. Based on the SSR (simple sequence repeat) analyses 46 of the 48 investigated varieties were identified. Even 'Pinot blanc' and 'Pinot gris' cultivars belonging to the same conculta (Pinot) could be differentiated in their VMC4A1 locus.

Original languageEnglish
Pages (from-to)213-221
Number of pages9
JournalScientia Horticulturae
Volume120
Issue number2
DOIs
Publication statusPublished - Apr 2 2009

Fingerprint

Vitis vinifera
isozymes
grapes
germplasm
microsatellite repeats
enzymes
loci
transaminases
catechol oxidase
acid phosphatase
glutamates
biomarkers
peroxidase
taxonomy
genetic variation
genetic markers
stems
cultivars
sampling

Keywords

  • Cultivar identification
  • Grape
  • SSR
  • Taxonomy
  • Vitis

ASJC Scopus subject areas

  • Horticulture

Cite this

Isoenzyme and microsatellite analysis of Vitis vinifera L. varieties from the Hungarian grape germplasm. / Jahnke, G.; Májer, J.; Lakatos, A.; Molnár, J. Györffyné; Deák, E.; Stefanovits-Banyai, E.; Varga, P.

In: Scientia Horticulturae, Vol. 120, No. 2, 02.04.2009, p. 213-221.

Research output: Contribution to journalArticle

Jahnke, G. ; Májer, J. ; Lakatos, A. ; Molnár, J. Györffyné ; Deák, E. ; Stefanovits-Banyai, E. ; Varga, P. / Isoenzyme and microsatellite analysis of Vitis vinifera L. varieties from the Hungarian grape germplasm. In: Scientia Horticulturae. 2009 ; Vol. 120, No. 2. pp. 213-221.
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