Iscom of viral envelope proteins protects against Aujeszky's disease

B. Morein, S. Belák, T. Soós, M. Rusvai, B. S. McGwire, K. Bognár

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

An immunostimulating complex (iscom) containing the envelope proteins of pseudorabies virus (PRV) was prepared and its efficacy was evaluated in two experiments on sheep. In the first experiment, sheep were intramuscularly (i.m.) or intradermally (i.d.) vaccinated with PRV iscom doses varying between 1 and 81 μg. The vaccination was repeated on Day 21 and the animals were exposed to challenge infection by subcutaneous inoculation of 1000 TCID50 of the virulent Phylaxia strain on Day 35 after first vaccination. In the second experiment, sheep were i.m. vaccinated with single doses of iscom varying between 1 and 27 μg and challenge-infected on Day 14. It was found that: (1) the i.d. administration of PRV iscom has no advantage over i.m. administration (2); a single dose of ≥ 3 μg of PRV iscom provided protection against the disease. In immunoblots, viral proteins of molecular masses 120, 109, 55, 53 and 32 kDa were detected with the sera obtained from iscom-vaccinated and subsequently challenge-infected sheep, but not with sera from sheep which were iscom-vaccinated only. The above findings indicated that: (1) by using iscom technology, potent subunit vaccines can be prepared to prevent Aujeszky's disease; (2) the selective incorporation of viral envelope proteins into iscoms gives the opportunity to discriminate between iscom-vaccinated and naturally infected animals.

Original languageEnglish
Pages (from-to)143-154
Number of pages12
JournalVeterinary Microbiology
Volume20
Issue number2
DOIs
Publication statusPublished - 1989

Fingerprint

ISCOMs
Pseudorabies
Viral Envelope Proteins
Aujeszky disease
Suid herpesvirus 1
sheep
Suid Herpesvirus 1
Sheep
vaccination
dosage
subunit vaccines
viral proteins
Vaccination
animals
viral envelope proteins
molecular weight
Subunit Vaccines
Viral Proteins
Serum
infection

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

Iscom of viral envelope proteins protects against Aujeszky's disease. / Morein, B.; Belák, S.; Soós, T.; Rusvai, M.; McGwire, B. S.; Bognár, K.

In: Veterinary Microbiology, Vol. 20, No. 2, 1989, p. 143-154.

Research output: Contribution to journalArticle

Morein, B, Belák, S, Soós, T, Rusvai, M, McGwire, BS & Bognár, K 1989, 'Iscom of viral envelope proteins protects against Aujeszky's disease', Veterinary Microbiology, vol. 20, no. 2, pp. 143-154. https://doi.org/10.1016/0378-1135(89)90037-0
Morein, B. ; Belák, S. ; Soós, T. ; Rusvai, M. ; McGwire, B. S. ; Bognár, K. / Iscom of viral envelope proteins protects against Aujeszky's disease. In: Veterinary Microbiology. 1989 ; Vol. 20, No. 2. pp. 143-154.
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