Ischemic limb preconditioning downregulates systemic inflammatory activation

Andrea Szabó, Renáta Varga, Margit Keresztes, C. Vízler, István Németh, Zsolt Rázga, M. Borós

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We examined local and systemic antiinflammatory consequences of ischemic preconditioning (IPC) in a rat model of limb ischemia-reperfusion (I-R) by characterizing the leukocyte-endothelial interactions in the periosteum and the expression of adhesion molecules playing a role in leukocyte-mediated inflammatory processes. IPC induction (2 cycles of 10 min of complete limb ischemia and 10 min of reperfusion) was followed by 60 min of ischemia/180 min of reperfusion or sham-operation. Data were compared with those on animals subjected to I-R and sham-operation. Neutrophil leukocyte-endothelial cell interactions (intravital videomicroscopy), intravascular neutrophil activation (CD11b expression changes by flow cytometry), and soluble and tissue intercellular adhesion molecule-1 (ICAM-1; ELISA and immunohistochemistry, respectively) expressions were assessed. I-R induced enhanced leukocyte rolling and adherence in the periosteal postcapillary venules after 120 and 180 min of reperfusion. This was associated with a significantly enhanced CD11b expression (by ∼80% and 72%, respectively) and moderately increased soluble and periosteal ICAM-1 expressions. IPC prevented the I-R-induced increases in leukocyte adherence and CD11b expression without influencing the soluble and tissue ICAM-1 levels. The results show that limb IPC exerts not only local, but distant antiinflammatory effects through significant modulation of neutrophil recruitment.

Original languageEnglish
Pages (from-to)897-902
Number of pages6
JournalJournal of Orthopaedic Research
Volume27
Issue number7
DOIs
Publication statusPublished - Jul 2009

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Ischemic Preconditioning
Reperfusion
Down-Regulation
Extremities
Ischemia
Intercellular Adhesion Molecule-1
Leukocytes
Anti-Inflammatory Agents
Tissue Adhesions
Leukocyte Rolling
Video Microscopy
Periosteum
Neutrophil Activation
Venules
Neutrophil Infiltration
Cell Communication
Flow Cytometry
Neutrophils
Endothelial Cells
Enzyme-Linked Immunosorbent Assay

Keywords

  • CD11b
  • ICAM-1
  • Microcirculation
  • Neutrophil leukocyte
  • Periosteum

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Ischemic limb preconditioning downregulates systemic inflammatory activation. / Szabó, Andrea; Varga, Renáta; Keresztes, Margit; Vízler, C.; Németh, István; Rázga, Zsolt; Borós, M.

In: Journal of Orthopaedic Research, Vol. 27, No. 7, 07.2009, p. 897-902.

Research output: Contribution to journalArticle

Szabó, Andrea ; Varga, Renáta ; Keresztes, Margit ; Vízler, C. ; Németh, István ; Rázga, Zsolt ; Borós, M. / Ischemic limb preconditioning downregulates systemic inflammatory activation. In: Journal of Orthopaedic Research. 2009 ; Vol. 27, No. 7. pp. 897-902.
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