Ischemic heart disease induce upregulation of endothelin receptor mRNA in human coronary arteries

Angelica Wackenfors, Malin Emilson, Richard Ingemansson, Tibor Hortobagyi, Delia Szok, Janos Tajti, Laszlo Vecsei, Lars Edvinsson, Malin Malmsjö

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Endothelin has been implicated in the pathogenesis of ischemic heart disease and congestive heart failure. The aims were to quantify endothelin type A (ETA) and type B (ETB) receptor mRNA levels in human coronary arteries from patients with ischemic heart disease, congestive heart failure and controls using real-time polymerase chain reaction (real-time PCR). In addition, the suitability of organ culture as a model mimicking endothelin receptor changes in cardiovascular disease was evaluated by in vitro pharmacology and real-time PCR. Endothelin ETA and ETB receptor mRNA levels were significantly higher in arteries from patients with ischemic heart disease (0.23±0.04 and 0.35±0.06) as compared to congestive heart failure (0.09±0.02 and 0.07±0.01) and controls (0.08±0.02 and 0.08±0.01). After organ culture, the endothelin ETB receptor mRNA levels were elevated, and the sarafotoxin 6c-induced vasoconstriction was more efficacious. Increased endothelin receptor activity may contribute to the increased vascular tone and development of atherosclerotic disease in ischemic heart disease in man.

Original languageEnglish
Pages (from-to)103-109
Number of pages7
JournalEuropean Journal of Pharmacology
Volume484
Issue number1
DOIs
Publication statusPublished - Jan 19 2004

Keywords

  • Cardiovascular disease
  • Coronary artery
  • Endothelin
  • Endothelin receptor
  • Gene expression
  • Vasoconstriction

ASJC Scopus subject areas

  • Pharmacology

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