Ischaemic postconditioning reduces peroxide formation, cytokine expression and leukocyte activation in reperfusion injury after abdominal aortic surgery in rat model

L. Sínay, M. Kürthy, Sz Horváth, E. Arató, M. Shafiei, J. Lantos, S. Ferencz, A. Bátor, B. Balatonyi, Zs Verzár, B. Süto, L. Kollár, G. Wéber, E. Rőth, G. Jancsó

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Objective: We studied the protective effects of ischaemic postconditioning (PS) on ischemia-reperfusion injury of the lower extremities in a rat model of abdominal aortic intervention. We aimed to examine the evoked oxidative stress, cytokine expression and leukocyte activation after revascularisation surgery. Methods: Anesthetized animals (48 Whistar rats) underwent a 60 min infrarenal aorta cross-clamping. After the ischaemic period, an intermittent 4 times 15 s reperfusion - 15 seconds ischaemic episodes - were applied (ischaemic postconditioning: group PS). Then we started a 120 min reperfusion in the aorta. In untreated group animals underwent a long ischaemia (60 min) and the following reperfusion (group IR). Peripherial blood samples were collected before operation, and in early (5, 10, 15, 30, 60 and 120 min) reperfusion periods. Serum peroxide level, TNF-alpha concentration, myeloperoxidase (MPO) activity and PMA-induced leukocyte ROS production were measured. Results: In PS group, plasma peroxide level elevation was significantly lower in very early reperfusion (5-30 min) comparing to non-conditioned IR group (10.04±1.9 μM/l vs. 16.91±3.67 μM/l, p3 cells vs. 4.63±0.69 AU/103 cells). Conclusions: Ischaemic postconditioning could reduce ROI production after IR in early reperfusion period, thus limiting ROI mediated tissue lesion, cytokine-leukocyte activation and inflammatory responses. PS seems to be an effective tool in vascular surgery to reduce reperfusion injuries after revascularization interventions.

Original languageEnglish
Pages (from-to)133-142
Number of pages10
JournalClinical Hemorheology and Microcirculation
Volume40
Issue number2
DOIs
Publication statusPublished - 2008

Fingerprint

Ischemic Postconditioning
Peroxides
Reperfusion Injury
Reperfusion
Leukocytes
Cytokines
Aorta
Constriction
Peroxidase
Blood Vessels
Lower Extremity
Oxidative Stress
Ischemia
Tumor Necrosis Factor-alpha
Serum

Keywords

  • Inflammatory response
  • Leukocyte
  • Postconditioning
  • Reactive oxygen species
  • Reperfusion injury
  • TNF-alpha
  • Vascular surgery

ASJC Scopus subject areas

  • Hematology
  • Physiology
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

Ischaemic postconditioning reduces peroxide formation, cytokine expression and leukocyte activation in reperfusion injury after abdominal aortic surgery in rat model. / Sínay, L.; Kürthy, M.; Horváth, Sz; Arató, E.; Shafiei, M.; Lantos, J.; Ferencz, S.; Bátor, A.; Balatonyi, B.; Verzár, Zs; Süto, B.; Kollár, L.; Wéber, G.; Rőth, E.; Jancsó, G.

In: Clinical Hemorheology and Microcirculation, Vol. 40, No. 2, 2008, p. 133-142.

Research output: Contribution to journalArticle

Sínay, L. ; Kürthy, M. ; Horváth, Sz ; Arató, E. ; Shafiei, M. ; Lantos, J. ; Ferencz, S. ; Bátor, A. ; Balatonyi, B. ; Verzár, Zs ; Süto, B. ; Kollár, L. ; Wéber, G. ; Rőth, E. ; Jancsó, G. / Ischaemic postconditioning reduces peroxide formation, cytokine expression and leukocyte activation in reperfusion injury after abdominal aortic surgery in rat model. In: Clinical Hemorheology and Microcirculation. 2008 ; Vol. 40, No. 2. pp. 133-142.
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abstract = "Objective: We studied the protective effects of ischaemic postconditioning (PS) on ischemia-reperfusion injury of the lower extremities in a rat model of abdominal aortic intervention. We aimed to examine the evoked oxidative stress, cytokine expression and leukocyte activation after revascularisation surgery. Methods: Anesthetized animals (48 Whistar rats) underwent a 60 min infrarenal aorta cross-clamping. After the ischaemic period, an intermittent 4 times 15 s reperfusion - 15 seconds ischaemic episodes - were applied (ischaemic postconditioning: group PS). Then we started a 120 min reperfusion in the aorta. In untreated group animals underwent a long ischaemia (60 min) and the following reperfusion (group IR). Peripherial blood samples were collected before operation, and in early (5, 10, 15, 30, 60 and 120 min) reperfusion periods. Serum peroxide level, TNF-alpha concentration, myeloperoxidase (MPO) activity and PMA-induced leukocyte ROS production were measured. Results: In PS group, plasma peroxide level elevation was significantly lower in very early reperfusion (5-30 min) comparing to non-conditioned IR group (10.04±1.9 μM/l vs. 16.91±3.67 μM/l, p3 cells vs. 4.63±0.69 AU/103 cells). Conclusions: Ischaemic postconditioning could reduce ROI production after IR in early reperfusion period, thus limiting ROI mediated tissue lesion, cytokine-leukocyte activation and inflammatory responses. PS seems to be an effective tool in vascular surgery to reduce reperfusion injuries after revascularization interventions.",
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T1 - Ischaemic postconditioning reduces peroxide formation, cytokine expression and leukocyte activation in reperfusion injury after abdominal aortic surgery in rat model

AU - Sínay, L.

AU - Kürthy, M.

AU - Horváth, Sz

AU - Arató, E.

AU - Shafiei, M.

AU - Lantos, J.

AU - Ferencz, S.

AU - Bátor, A.

AU - Balatonyi, B.

AU - Verzár, Zs

AU - Süto, B.

AU - Kollár, L.

AU - Wéber, G.

AU - Rőth, E.

AU - Jancsó, G.

PY - 2008

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N2 - Objective: We studied the protective effects of ischaemic postconditioning (PS) on ischemia-reperfusion injury of the lower extremities in a rat model of abdominal aortic intervention. We aimed to examine the evoked oxidative stress, cytokine expression and leukocyte activation after revascularisation surgery. Methods: Anesthetized animals (48 Whistar rats) underwent a 60 min infrarenal aorta cross-clamping. After the ischaemic period, an intermittent 4 times 15 s reperfusion - 15 seconds ischaemic episodes - were applied (ischaemic postconditioning: group PS). Then we started a 120 min reperfusion in the aorta. In untreated group animals underwent a long ischaemia (60 min) and the following reperfusion (group IR). Peripherial blood samples were collected before operation, and in early (5, 10, 15, 30, 60 and 120 min) reperfusion periods. Serum peroxide level, TNF-alpha concentration, myeloperoxidase (MPO) activity and PMA-induced leukocyte ROS production were measured. Results: In PS group, plasma peroxide level elevation was significantly lower in very early reperfusion (5-30 min) comparing to non-conditioned IR group (10.04±1.9 μM/l vs. 16.91±3.67 μM/l, p3 cells vs. 4.63±0.69 AU/103 cells). Conclusions: Ischaemic postconditioning could reduce ROI production after IR in early reperfusion period, thus limiting ROI mediated tissue lesion, cytokine-leukocyte activation and inflammatory responses. PS seems to be an effective tool in vascular surgery to reduce reperfusion injuries after revascularization interventions.

AB - Objective: We studied the protective effects of ischaemic postconditioning (PS) on ischemia-reperfusion injury of the lower extremities in a rat model of abdominal aortic intervention. We aimed to examine the evoked oxidative stress, cytokine expression and leukocyte activation after revascularisation surgery. Methods: Anesthetized animals (48 Whistar rats) underwent a 60 min infrarenal aorta cross-clamping. After the ischaemic period, an intermittent 4 times 15 s reperfusion - 15 seconds ischaemic episodes - were applied (ischaemic postconditioning: group PS). Then we started a 120 min reperfusion in the aorta. In untreated group animals underwent a long ischaemia (60 min) and the following reperfusion (group IR). Peripherial blood samples were collected before operation, and in early (5, 10, 15, 30, 60 and 120 min) reperfusion periods. Serum peroxide level, TNF-alpha concentration, myeloperoxidase (MPO) activity and PMA-induced leukocyte ROS production were measured. Results: In PS group, plasma peroxide level elevation was significantly lower in very early reperfusion (5-30 min) comparing to non-conditioned IR group (10.04±1.9 μM/l vs. 16.91±3.67 μM/l, p3 cells vs. 4.63±0.69 AU/103 cells). Conclusions: Ischaemic postconditioning could reduce ROI production after IR in early reperfusion period, thus limiting ROI mediated tissue lesion, cytokine-leukocyte activation and inflammatory responses. PS seems to be an effective tool in vascular surgery to reduce reperfusion injuries after revascularization interventions.

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KW - Leukocyte

KW - Postconditioning

KW - Reactive oxygen species

KW - Reperfusion injury

KW - TNF-alpha

KW - Vascular surgery

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