Is there penetration of titania nanoparticles in sunscreens through skin? A comparative electron and ion microscopy study

Etienne Gontier, Maria Dolores Ynsa, Tamás Bíró, Janos Hunyadi, Borbala Kiss, Krisztián Gáspár, Teresa Pinheiro, Joao Nuno Silva, Paulo Filipe, Jerzy Stachura, Wojciech Dabros, Tilo Reinert, Tilman Butz, Philippe Moretto, Jean Etienne Surlève-Bazeille

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

We report on a comparative study by Transmission Electron Microscopy (HRTEM) and Scanning Transmission Ion Microscopy (STIM) combined with Rutherford Backscattering Spectrometry (RBS) and Particle Induced X-Ray Emission (PIXE) on ultra-thin and thin cross-sections, respectively, of various skin samples (porcine skin, healthy human skin, human skin grafted on a severe combined immuno-deficient mouse model) to which we applied topically various formulations containing titanium dioxide (TiO2) nanoparticles with primary particle sizes in the range from 20-100 nm. Whereas the HRTEM and STIM/PIXE images reveal clear differences - mainly related to the different thickness of the cross-sections - they unambiguously show that penetration of TiO2 nanoparticles is restricted to the topmost 3-5 corneocyte layers of the stratum corneum (SC).

Original languageEnglish
Pages (from-to)218-231
Number of pages14
JournalNanotoxicology
Volume2
Issue number4
DOIs
Publication statusPublished - Nov 2008

Keywords

  • Electron microscopy
  • Ion microscopy
  • Mammalian skin
  • TiO2 nanoparticles

ASJC Scopus subject areas

  • Biomedical Engineering
  • Toxicology

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    Gontier, E., Ynsa, M. D., Bíró, T., Hunyadi, J., Kiss, B., Gáspár, K., Pinheiro, T., Silva, J. N., Filipe, P., Stachura, J., Dabros, W., Reinert, T., Butz, T., Moretto, P., & Surlève-Bazeille, J. E. (2008). Is there penetration of titania nanoparticles in sunscreens through skin? A comparative electron and ion microscopy study. Nanotoxicology, 2(4), 218-231. https://doi.org/10.1080/17435390802538508