Is there a role for the tumor cell integrin αIIbβ3 and cytoskeleton in tumor cell-platelet interaction?

Hemi Chopra, Jozsef Timar, X. Rong, Irma M. Grossi, James S. Hatfield, Suzanne E.G. Fligiel, Catherine A. Finch, John D. Taylor, Kenneth V. Honn

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37 Citations (Scopus)


In vitro tumor cell-platelet interaction was examined using B16 amelanotic (B16a) melanoma cells. These tumor cells express the αIIbβ3-type cytoadhesin. Aggregation studies demonstrated that tumor cell surface αIIbβ3 mediates the recognition of platelets since pretreatment of tumor cells with antibody against αIIbβ3 prevents platelet-tumor cell interaction as well as platelet activation measured by aggregometry, platelet eicosanoid metabolism and ultrastructural analysis. In B16a cells, disruption of the microfilaments and intermediate filaments inhibits mobility of αIIbβ3 on the cell surface. Microtubules do not play a role in receptor mobility, because B16a cells do not possess well-defined microtubules in interphase and colchicine does not affect receptor mobility. Disruption of microfilaments or intermediate filaments results in an inhibition of tumor cell-platelet interaction as evidenced by aggregometry studies and ultrastructural analysis. We suggest that platelet interaction with tumor cells begins with αIIbβ3-mediated receptor recognition followed by not only platelet activation but also microfilament- and vimentin intermediate filament-dependent tumor cell activation.

Original languageEnglish
Pages (from-to)125-137
Number of pages13
JournalClinical & Experimental Metastasis
Issue number2
Publication statusPublished - Mar 1 1992


  • B16a melanoma
  • cytoskeleton
  • intermediate filaments
  • microfilaments
  • tumor cell-induced platelet aggregation
  • αβ integrin

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Chopra, H., Timar, J., Rong, X., Grossi, I. M., Hatfield, J. S., Fligiel, S. E. G., Finch, C. A., Taylor, J. D., & Honn, K. V. (1992). Is there a role for the tumor cell integrin αIIbβ3 and cytoskeleton in tumor cell-platelet interaction? Clinical & Experimental Metastasis, 10(2), 125-137.