Is the early or delayed remote ischemic preconditioning the more effective from a microcirculatory and histological point of view in a rat model of partial liver ischemia-reperfusion?

Zsuzsanna Magyar, Gabor Varga, Anita Mester, Souleiman Ghanem, Viktoria Somogyi, Bence Tanczos, Adam Deak, Laszlo Bidiga, Katalin Peto, N. Németh

Research output: Contribution to journalArticle

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Abstract

Purpose: To compare early-and late-effect remote ischemic preconditioning (RIPC) by analyzing the microcirculatory, hemodynamic and histological changes in partial liver ischemia-reperfusion of rats. Methods: 60-minute partial liver ischemia followed by 120-minute reperfusion was performed without (Control group, n=7) or with preconditioning. In RIPC groups a tourniquet was applied around the left thigh using 3 cycles of 10-minute ischemia/10-minute reperfusion, one (RIPC-1, n=7) or twenty-four hours (RIPC-24, n=7) before I/R. Hemodynamic and microcirculatory measurements were performed before and after ischemia and in 30th, 60th and 120th minute of reperfusion and histological examination at the end of reperfusion. Results: Blood pressure decreased in all groups followed by biphasic changes in Control group. In RIPC groups R120 values returned almost to normal. Heart rate increased in Control and RIPC-1 groups at R120, while RIPC-24 did not show significant changes. Microcirculation of non-ischemic liver stayed constant in Control and showed significant changes in RIPC-24 group, while in ischemic liver elevated by R120 in all groups. RIPC didn’t reduce histological alterations. Conclusion: Considering the survival and the results, both remote ischemic preconditioning protocols had beneficial effect in hepatic ischemia-reperfusion, however the histopathological findings were controversial.

Original languageEnglish
Pages (from-to)597-608
Number of pages12
JournalActa Cirurgica Brasileira
Volume33
Issue number7
DOIs
Publication statusPublished - Jul 1 2018

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Ischemic Preconditioning
Reperfusion
Ischemia
Liver
Hemodynamics
Tourniquets
Control Groups
Microcirculation
Thigh
Heart Rate
Blood Pressure

Keywords

  • Histology
  • Ischemia
  • Ischemic preconditioning
  • Liver
  • Microcirculation
  • Rats
  • Reperfusion

ASJC Scopus subject areas

  • Surgery

Cite this

Is the early or delayed remote ischemic preconditioning the more effective from a microcirculatory and histological point of view in a rat model of partial liver ischemia-reperfusion? / Magyar, Zsuzsanna; Varga, Gabor; Mester, Anita; Ghanem, Souleiman; Somogyi, Viktoria; Tanczos, Bence; Deak, Adam; Bidiga, Laszlo; Peto, Katalin; Németh, N.

In: Acta Cirurgica Brasileira, Vol. 33, No. 7, 01.07.2018, p. 597-608.

Research output: Contribution to journalArticle

Magyar, Zsuzsanna ; Varga, Gabor ; Mester, Anita ; Ghanem, Souleiman ; Somogyi, Viktoria ; Tanczos, Bence ; Deak, Adam ; Bidiga, Laszlo ; Peto, Katalin ; Németh, N. / Is the early or delayed remote ischemic preconditioning the more effective from a microcirculatory and histological point of view in a rat model of partial liver ischemia-reperfusion?. In: Acta Cirurgica Brasileira. 2018 ; Vol. 33, No. 7. pp. 597-608.
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T1 - Is the early or delayed remote ischemic preconditioning the more effective from a microcirculatory and histological point of view in a rat model of partial liver ischemia-reperfusion?

AU - Magyar, Zsuzsanna

AU - Varga, Gabor

AU - Mester, Anita

AU - Ghanem, Souleiman

AU - Somogyi, Viktoria

AU - Tanczos, Bence

AU - Deak, Adam

AU - Bidiga, Laszlo

AU - Peto, Katalin

AU - Németh, N.

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Purpose: To compare early-and late-effect remote ischemic preconditioning (RIPC) by analyzing the microcirculatory, hemodynamic and histological changes in partial liver ischemia-reperfusion of rats. Methods: 60-minute partial liver ischemia followed by 120-minute reperfusion was performed without (Control group, n=7) or with preconditioning. In RIPC groups a tourniquet was applied around the left thigh using 3 cycles of 10-minute ischemia/10-minute reperfusion, one (RIPC-1, n=7) or twenty-four hours (RIPC-24, n=7) before I/R. Hemodynamic and microcirculatory measurements were performed before and after ischemia and in 30th, 60th and 120th minute of reperfusion and histological examination at the end of reperfusion. Results: Blood pressure decreased in all groups followed by biphasic changes in Control group. In RIPC groups R120 values returned almost to normal. Heart rate increased in Control and RIPC-1 groups at R120, while RIPC-24 did not show significant changes. Microcirculation of non-ischemic liver stayed constant in Control and showed significant changes in RIPC-24 group, while in ischemic liver elevated by R120 in all groups. RIPC didn’t reduce histological alterations. Conclusion: Considering the survival and the results, both remote ischemic preconditioning protocols had beneficial effect in hepatic ischemia-reperfusion, however the histopathological findings were controversial.

AB - Purpose: To compare early-and late-effect remote ischemic preconditioning (RIPC) by analyzing the microcirculatory, hemodynamic and histological changes in partial liver ischemia-reperfusion of rats. Methods: 60-minute partial liver ischemia followed by 120-minute reperfusion was performed without (Control group, n=7) or with preconditioning. In RIPC groups a tourniquet was applied around the left thigh using 3 cycles of 10-minute ischemia/10-minute reperfusion, one (RIPC-1, n=7) or twenty-four hours (RIPC-24, n=7) before I/R. Hemodynamic and microcirculatory measurements were performed before and after ischemia and in 30th, 60th and 120th minute of reperfusion and histological examination at the end of reperfusion. Results: Blood pressure decreased in all groups followed by biphasic changes in Control group. In RIPC groups R120 values returned almost to normal. Heart rate increased in Control and RIPC-1 groups at R120, while RIPC-24 did not show significant changes. Microcirculation of non-ischemic liver stayed constant in Control and showed significant changes in RIPC-24 group, while in ischemic liver elevated by R120 in all groups. RIPC didn’t reduce histological alterations. Conclusion: Considering the survival and the results, both remote ischemic preconditioning protocols had beneficial effect in hepatic ischemia-reperfusion, however the histopathological findings were controversial.

KW - Histology

KW - Ischemia

KW - Ischemic preconditioning

KW - Liver

KW - Microcirculation

KW - Rats

KW - Reperfusion

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