Is an amphiphilic region responsible for the haemolytic activity of Bacillus thuringiensis toxin?

E. Szabo, J. Murvai, P. Fabian, F. Fabian, M. Hollósi, J. Kajtar, Z. Buzas, M. Sajgo, S. Pongor, B. Asboth

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The amino acid sequence of the 27 kDa protein responsible for the haemolytic activity of Bacilus thuringiensis subsp. israelensis toxin has been aanlysed by secondary structure prediction, helical wheel/net diagrams and molecular mechanics calculations. We found that segment 116-126 presumably forms a strongly amphiphilic α-helix. This is supported by the findings that the synthesized segment 116-126 (a) has a significant α-helical content in water, and (b) displays an in vitro haemolytic activity comparable to that of bee venom peptide melittin. As segment 116-126 is present in the haemolyzing, but not present in the non-haemolyzing proteins from B. thuringiensis toxins, we suggest that this segment is responsible for the lytic potential of the B. thuringiensis subsp. israelensis protein.

Original languageEnglish
Pages (from-to)527-532
Number of pages6
JournalInternational Journal of Peptide and Protein Research
Volume42
Issue number6
Publication statusPublished - 1993

Fingerprint

Bacillus thuringiensis
Bacilli
Melitten
Bee Venoms
Molecular mechanics
Mechanics
Amino Acid Sequence
Wheels
Proteins
Amino Acids
Peptides
Water
IgA receptor
In Vitro Techniques

Keywords

  • Amphipathic helix
  • Bacillus thuringiensis subsp. israelensis
  • Circular dichroism spectrum
  • Haemolysis
  • Secondary structure prediction
  • Synthetic oligopeptide

ASJC Scopus subject areas

  • Biochemistry

Cite this

Is an amphiphilic region responsible for the haemolytic activity of Bacillus thuringiensis toxin? / Szabo, E.; Murvai, J.; Fabian, P.; Fabian, F.; Hollósi, M.; Kajtar, J.; Buzas, Z.; Sajgo, M.; Pongor, S.; Asboth, B.

In: International Journal of Peptide and Protein Research, Vol. 42, No. 6, 1993, p. 527-532.

Research output: Contribution to journalArticle

Szabo, E, Murvai, J, Fabian, P, Fabian, F, Hollósi, M, Kajtar, J, Buzas, Z, Sajgo, M, Pongor, S & Asboth, B 1993, 'Is an amphiphilic region responsible for the haemolytic activity of Bacillus thuringiensis toxin?', International Journal of Peptide and Protein Research, vol. 42, no. 6, pp. 527-532.
Szabo, E. ; Murvai, J. ; Fabian, P. ; Fabian, F. ; Hollósi, M. ; Kajtar, J. ; Buzas, Z. ; Sajgo, M. ; Pongor, S. ; Asboth, B. / Is an amphiphilic region responsible for the haemolytic activity of Bacillus thuringiensis toxin?. In: International Journal of Peptide and Protein Research. 1993 ; Vol. 42, No. 6. pp. 527-532.
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