Is an amphiphilic region responsible for the haemolytic activity of Bacillus thuringiensis toxin?

ERIKA SZABÓ, JÁNOS MURVAI, PÉTER FÁBIÁN, FERENC FÁBIÁN, MIKLÓS HOLLÓSI, JUDIT KaJTÁR, ZSUZSA BUZÁS, MIHÁLY SAJGÓ, SÁNDOR PONGOR, BENCE ASBÓTH

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The amino acid sequence of the 27 kDa protein responsible for the haemolytic activity of Bacillus thuringiensis subsp. israelensis toxin has been analysed by secondary structure prediction, helical wheel/net diagrams and molecular mechanics calculations. We found that segment 116–126 presumably forms a strongly amphiphilic α‐helix. This is supported by the findings that the synthesized segment 116‐126 (a) has a significant α‐helical content in water, and (b) displays an in vitro haemolytic activity comparable to that of bee venom peptide melittin. As segment 116‐126 is present in the haemolyzing, but not present in the non‐haemolyzing proteins from B. thuringiensis toxins, we suggest that this segment is responsible for the lytic potential of the B. thuringiensis subsp. israelensis protein.

Original languageEnglish
Pages (from-to)527-532
Number of pages6
JournalInternational journal of peptide and protein research
Volume42
Issue number6
DOIs
Publication statusPublished - Aug 1993

Keywords

  • Bacillus thuringiensis subsp. israelensis
  • amphipathic helix
  • circular dichroism spectrum
  • haemolysis
  • secondary structure prediction
  • synthetic oligopeptide

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Is an amphiphilic region responsible for the haemolytic activity of Bacillus thuringiensis toxin?'. Together they form a unique fingerprint.

  • Cite this

    SZABÓ, ERIKA., MURVAI, JÁNOS., FÁBIÁN, PÉTER., FÁBIÁN, FERENC., HOLLÓSI, MIKLÓS., KaJTÁR, JUDIT., BUZÁS, ZSUZSA., SAJGÓ, MIHÁLY., PONGOR, SÁNDOR., & ASBÓTH, BENCE. (1993). Is an amphiphilic region responsible for the haemolytic activity of Bacillus thuringiensis toxin? International journal of peptide and protein research, 42(6), 527-532. https://doi.org/10.1111/j.1399-3011.1993.tb00360.x